Narváez Diana M, Groot Helena, Diaz Sonia M, Palma Ruth Marien, Muñoz Nathalia, Cros Marie-Pierre, Hernández-Vargas Hector
Human Genetics Laboratory. Universidad de los Andes. Bogotá, Colombia.
Instituto Nacional de Salud (INS). Bogotá, Colombia.
Heliyon. 2017 Sep 12;3(9):e00400. doi: 10.1016/j.heliyon.2017.e00400. eCollection 2017 Sep.
Mercury (Hg) exposure is a public health concern due to its persistence in the environment and its high toxicity. Such toxicity has been associated with the generation of oxidative stress in occupationally exposed subjects, such as artisanal gold miners. In this study, we characterize occupational exposure to Hg by measuring blood, urine and hair levels, and investigate oxidative stress and DNA methylation associated with gold mining. To do this, samples from 53 miners and 36 controls were assessed. We show higher levels of oxidative stress marker 8-OHdG in the miners. Differences in and DNA methylation between gold miners and control group are present in peripheral blood leukocytes. methylation is positively correlated with 8-OHdG levels, while and methylation are positively correlated with Hg levels. These results suggest an effect of Hg on oxidative stress and DNA methylation in gold miners that may have an impact on miners' health.
由于汞（Hg）在环境中具有持久性且毒性很高，汞暴露成为一个公共卫生问题。这种毒性与职业暴露人群（如个体金矿开采者）体内氧化应激的产生有关。在本研究中，我们通过测量血液、尿液和头发中的汞含量来表征职业性汞暴露，并调查与金矿开采相关的氧化应激和DNA甲基化情况。为此，我们评估了53名矿工和36名对照者的样本。我们发现矿工体内氧化应激标志物8-羟基脱氧鸟苷（8-OHdG）的水平更高。金矿开采者与对照组在外周血白细胞中的[具体基因]和[具体基因]DNA甲基化存在差异。[具体基因]甲基化与8-OHdG水平呈正相关，而[具体基因]和[具体基因]甲基化与汞含量呈正相关。这些结果表明汞对金矿开采者的氧化应激和DNA甲基化有影响，这可能会对矿工的健康产生影响。
