Min G L, Hibbin J, Arthur C, Apperley J, Jeffreys A, Goldman J
MRC Leukaemia Unit, Royal Postgraduate Medical School, London.
Br J Haematol. 1988 Feb;68(2):195-201. doi: 10.1111/j.1365-2141.1988.tb06189.x.
Restriction fragment length polymorphisms can be used to distinguish blood and marrow cells from close relatives. We used two probes that recognize a series of dispersed and highly polymorphic tandem-repetitive minisatellite regions in the human genome that can be detected via a shared 10-15 base pair core sequence similar to the generalized recombination sequence (chi) of E. coli. We have studied the resulting individual-specific DNA fingerprints in 15 patients before and after allogeneic bone marrow transplantation performed for chronic myeloid leukaemia and in two patients transplanted for acute leukaemia. Early engraftment could be demonstrated at 3 weeks post-transplant based on the recognition of cells of donor origin. One patient who failed to engraft had only recipient type marrow cells 3 months post-transplant. Nine patients who relapsed after transplantation had only cells of recipient origin. In one patient who relapsed after transplantation with T-cell depleted donor marrow, fractionation studies showed that his T-cells at relapse were of recipient origin. We conclude that these minisatellite probes are valuable for characterizing the origin of different cell populations after marrow transplantation and could be useful for characterizing relapse when donor and recipient are of the same sex.
限制性片段长度多态性可用于区分近亲的血液和骨髓细胞。我们使用了两种探针，它们能识别人类基因组中一系列分散且高度多态的串联重复小卫星区域，这些区域可通过与大肠杆菌的通用重组序列（chi）相似的10 - 15个碱基对的共有核心序列来检测。我们研究了15例接受慢性髓性白血病同种异体骨髓移植的患者以及2例接受急性白血病移植的患者在移植前后产生的个体特异性DNA指纹图谱。基于对供体来源细胞的识别，可在移植后3周证明早期植入。一名未植入的患者在移植后3个月仅有受体型骨髓细胞。9例移植后复发的患者仅有受体来源的细胞。在一名接受去除T细胞的供体骨髓移植后复发的患者中，分级分离研究表明其复发时的T细胞是受体来源的。我们得出结论，这些小卫星探针对于骨髓移植后不同细胞群体的起源特征分析很有价值，并且在供体和受体为同性时，对于复发特征分析可能有用。
