鼠李糖乳杆菌 NCDC 298 与 FOS 联合对产肠毒素大肠埃希菌的活力和毒素产生的影响。
Effect of Lactobacillus rhamnosus NCDC 298 with FOS in Combination on Viability and Toxin Production of Enterotoxigenic Escherichia coli.
机构信息
TFSL, Dairy Microbiology Division, ICAR-National Dairy Research Institute, Karnal, Haryana, India.
出版信息
Probiotics Antimicrob Proteins. 2019 Mar;11(1):23-29. doi: 10.1007/s12602-017-9327-1.
The present study was to investigate the utilization of prebiotics by Lactobacillus rhamnosus NCDC 298 and its synergistic adversary effect on both population and production of heat-labile (LT) toxin in enterotoxigenic Escherichia coli (ETEC). To select suitable prebiotic in order to enhance functionality, its utilization and the prebiotic activity score was examined. Antivirulence effect on ETEC was inspected by its inactivation rate and heat-labile toxin production in presence of different synbiotic combination. L. rhamnosus NCDC 298 strain grown well on media supplemented with fructooligosaccharides (FOS) and galactooligosaccharides (GOS), whereas significant inactivation of ETEC was observed when FOS was added to the co-culture medium. Significant decrease in LT enterotoxin was seen through GM1 ganglioside enzyme linked immunoassay (GM1 ELISA), when ETEC has grown with L. rhamnosus NCDC 298 and FOS. Short-chain FOS proved to be the most effective substrate, improving antagonistic activity for L. rhamnosus NCDC 298. Both L. rhamnosus NCDC 298 with FOS can be used as an effective synbiotic combination for secretory antidiarrheal fermented dairy formulations.
本研究旨在探讨鼠李糖乳杆菌 NCDC 298 对益生元的利用及其对肠产毒性大肠杆菌（ETEC）群体和热不稳定（LT）毒素产量的协同拮抗作用。为了选择合适的益生元以增强其功能，研究了其利用率和益生元活性评分。通过不同的共生组合，检查了对 ETEC 的抗病毒作用，包括其失活率和 LT 毒素的产生。当在共培养培养基中添加果寡糖（FOS）和半乳糖寡糖（GOS）时，鼠李糖乳杆菌 NCDC 298 菌株在培养基中生长良好，而当添加 FOS 时，观察到对 ETEC 的显著失活。通过 GM1 神经节苷脂酶联免疫吸附试验（GM1 ELISA）观察到 LT 肠毒素显著减少，当 ETEC 与鼠李糖乳杆菌 NCDC 298 和 FOS 一起生长时。短链 FOS 被证明是最有效的底物，可提高鼠李糖乳杆菌 NCDC 298 的拮抗活性。含有 FOS 的鼠李糖乳杆菌 NCDC 298 均可作为一种有效的共生组合，用于分泌性抗腹泻发酵乳制品的配方。
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