Ge Jian-Bin, Lu Hong-Jian, Song Xin-Jian, Li Mei, Chen Dan-Dan, Wu Feng
The Second People's Hospital of Nantong, Nantong 226002, China.
Department of Pharmacology, College of Pharmaceutical Science, Soochow University, Suzhou 215123, China.
Zhongguo Zhong Yao Za Zhi. 2017 Jan;42(2):326-331. doi: 10.19540/j.cnki.cjcmm.20161222.016.
To observe the protective effects of Lycium barbarum polysaccharides (LBP) on cerebral ischemia reperfusion injury in mice and explore its mechanism. Common carotid artery thread was used to cause middle cerebral artery ischemia, and the thread was taken out after 2 h ischemia to achieve cerebral ischemia reperfusion injury in mice. Therefore, the transient middle cerebral artery occlusion (tMCAO) models were established to observe the effects of LBP (25,50, 100 mg•kg⁻¹) on neurological outcome, infarct size and water contents. HE staining was used to observe its effects on neurocytes of cerebral tissues in mice. Western blotting was used to evaluate the protein expression levels of NF-κB p65. ELISA was used to evaluate the levels of TNF-α, IL-6 and IL-1β in the serum. According to the results, LBP markedly improved neurologic deficits, and decreased infarct size and water contents at 24 h after reperfusion in mice. Pathological section of brain tissues also proved its protective effects on neurocytes. Western blot analysis indicated that LBP markedly down-regulated the protein level of NF-κB p65. ELISA indicated that LBP decreased the levels of TNF-α, IL-6 and IL-1β in the serum 24 h after reperfusion.In conclusion, LBP has protective effects on cerebral ischemia reperfusion injury in mice, and this effect may be associated with inhibiting NF-κB and inflammatory reactions.
观察枸杞多糖（LBP）对小鼠脑缺血再灌注损伤的保护作用并探讨其机制。采用颈总动脉线栓法造成大脑中动脉缺血，缺血2小时后抽出丝线以造成小鼠脑缺血再灌注损伤。因此，建立短暂性大脑中动脉闭塞（tMCAO）模型，观察LBP（25、50、100mg•kg⁻¹）对神经功能结局、梗死体积和含水量的影响。采用苏木精-伊红（HE）染色观察其对小鼠脑组织神经细胞的影响。采用蛋白质印迹法评估核因子-κB p65（NF-κB p65）的蛋白表达水平。采用酶联免疫吸附测定法（ELISA）评估血清中肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）和白细胞介素-1β（IL-1β）的水平。结果显示，LBP可显著改善小鼠再灌注后24小时的神经功能缺损，减小梗死体积并降低含水量。脑组织病理切片也证实了其对神经细胞的保护作用。蛋白质印迹分析表明，LBP可显著下调NF-κB p65的蛋白水平。ELISA表明，LBP可降低再灌注后24小时血清中TNF-α、IL-6和IL-1β的水平。综上所述，LBP对小鼠脑缺血再灌注损伤具有保护作用，且该作用可能与抑制NF-κB及炎症反应有关。
