辅酶Q10补充剂对多囊卵巢综合征患者胰岛素、脂质及炎症相关基因表达的影响。
The effects of coenzyme Q10 supplementation on gene expression related to insulin, lipid and inflammation in patients with polycystic ovary syndrome.
作者信息
Rahmani Elham, Jamilian Mehri, Samimi Mansooreh, Zarezade Mehrizi Maryam, Aghadavod Esmat, Akbari Elmira, Tamtaji Omid Reza, Asemi Zatollah
机构信息
a Department of Gynecology and Obstetrics, School of Medicine , Bushehr University of Medical Sciences , Bushehr , Iran.
b Endocrinology and Metabolism Research Center, Department of Gynecology and Obstetrics , School of Medicine, Arak University of Medical Sciences , Arak , Iran.
出版信息
Gynecol Endocrinol. 2018 Mar;34(3):217-222. doi: 10.1080/09513590.2017.1381680. Epub 2017 Sep 26.
OBJECTIVE
This research was conducted to assess the effects of coenzyme Q10 (CoQ10) intake on gene expression related to insulin, lipid and inflammation in subjects with polycystic ovary syndrome (PCOS).
METHODS
This randomized double-blind, placebo-controlled trial was conducted on 40 subjects diagnosed with PCOS. Subjects were randomly allocated into two groups to intake either 100 mg CoQ10 (n = 20) or placebo (n = 20) per day for 12 weeks. Gene expression related to insulin, lipid and inflammation were quantified in blood samples of PCOS women with RT-PCR method.
RESULTS
Results of RT-PCR shown that compared with the placebo, CoQ10 intake downregulated gene expression of oxidized low-density lipoprotein receptor 1 (LDLR) (p < 0.001) and upregulated gene expression of peroxisome proliferator-activated receptor gamma (PPAR-γ) (p = 0.01) in peripheral blood mononuclear cells of subjects with PCOS. In addition, compared to the placebo group, CoQ10 supplementation downregulated gene expression of interleukin-1 (IL-1) (p = 0.03), interleukin-8 (IL-8) (p = 0.001) and tumor necrosis factor alpha (TNF-α) (p < 0.001) in peripheral blood mononuclear cells of subjects with PCOS.
CONCLUSIONS
Overall, CoQ10 intake for 12 weeks in PCOS women significantly improved gene expression of LDLR, PPAR-γ, IL-1, IL-8 and TNF-α.
目的
本研究旨在评估多囊卵巢综合征（PCOS）患者摄入辅酶Q10（CoQ10）对胰岛素、脂质和炎症相关基因表达的影响。
方法
本随机双盲、安慰剂对照试验对40例诊断为PCOS的受试者进行。受试者被随机分为两组，每天分别摄入100毫克CoQ10（n = 20）或安慰剂（n = 20），持续12周。采用逆转录聚合酶链反应（RT-PCR）方法对PCOS女性血液样本中胰岛素、脂质和炎症相关基因表达进行定量分析。
结果
RT-PCR结果显示，与安慰剂相比，PCOS受试者外周血单个核细胞中，CoQ10摄入下调了氧化型低密度脂蛋白受体1（LDLR）的基因表达（p < 0.001），上调了过氧化物酶体增殖物激活受体γ（PPAR-γ）的基因表达（p = 0.01）。此外，与安慰剂组相比，CoQ10补充剂下调了PCOS受试者外周血单个核细胞中白细胞介素-1（IL-1）（p = 0.03）、白细胞介素-8（IL-8）（p = 0.001）和肿瘤坏死因子α（TNF-α）（p < 0.001）的基因表达。
结论
总体而言，PCOS女性摄入CoQ10 12周可显著改善LDLR、PPAR-γ、IL-1、IL-8和TNF-α的基因表达。
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