Findley H W, Kennedy M S, Lui V K, Ragab A H
Exp Hematol. 1979 May;7(5):259-63.
Acute lymphoblastic leukemia (ALL) in children is often characterized by defective granulopoiesis during initial and relapse stages of the disease, resulting in absolute neutropenia in vivo and in low or absent production of granulocyte-macrophage colonies in vitro. The purpose of this study was to determine if serum from leukemic children with ALL could inhibit normal granulopoiesis. Several concentrations of serum from 12 leukemic patients were mixed with normal bone marrow cells and co-cultured by the double layer agar technique. Also, serum from normal individuals and from ALL patients in remission and relapse was examined for inhibitors. Cultures without serum served as controls. The majority of ALL (initial, relapse and remission) and normal sera either stimulated or had no effect on colony formation. These groups also had similar percentages of inhibitory sera, with the exception of the somewhat higher levels of inhibition found in the remission group. Thus, ALL serum resembled normal serum in its effect on in vitro granulopoiesis at the committed granulocyte-macrophage stem cell level. It is therefore unlikely that inhibition of in vivo granulopoiesis at the committed level by serum inhibitors is responsible for ALL-associated neutropenia.
儿童急性淋巴细胞白血病（ALL）在疾病的初始阶段和复发阶段常表现为粒细胞生成缺陷，导致体内绝对中性粒细胞减少，体外粒细胞 - 巨噬细胞集落生成减少或缺乏。本研究的目的是确定ALL白血病患儿的血清是否会抑制正常粒细胞生成。将12例白血病患者的几种浓度血清与正常骨髓细胞混合，并通过双层琼脂技术进行共培养。此外，还检测了正常个体以及ALL缓解期和复发期患者血清中的抑制剂。无血清培养物作为对照。大多数ALL（初始、复发和缓解期）血清以及正常血清对集落形成要么有刺激作用，要么无影响。这些组中抑制性血清的百分比也相似，只是缓解组中发现的抑制水平略高。因此，ALL血清在定向粒细胞 - 巨噬细胞干细胞水平对体外粒细胞生成的影响与正常血清相似。所以，血清抑制剂在定向水平对体内粒细胞生成的抑制不太可能是ALL相关中性粒细胞减少的原因。
