Ma Yun, Li Zhiwei, Yuan Mei, Chen Linhua, Zhou Shanshan
a Key Laboratory of Microbial Technology for Industrial Pollution Control of Zhejiang Province, College of Environment , Zhejiang University of Technology , Hangzhou , People's Republic of China.
b Environmental Science Research Institute of Taizhou City , Taizhou , China.
J Environ Sci Health B. 2017 Nov 2;52(11):796-801. doi: 10.1080/03601234.2017.1356163. Epub 2017 Sep 26.
In this study, a bacterial strain, CH-1, capable of degrading 3-bromocarbazole (3-BCZ) was isolated from a polluted soil. Based on its physio-biochemical characteristics and 16S rRNA genes, strain CH-1 was identified as a Stenotrophomonas sp. Strain CH-1 was able to degrade 70% of 50 mg/L 3-BCZ within 8 d at pH 7.0 and 30°C in mineral salt medium (MSM). During the process, the main intermediate metabolite was identified as (2E, 4Z)-6-(2-amino-5-bromophenyl)-2-hydroxy-6-oxhexa-2, 4-dienoic by gas (2E, 4Z)-6-(2-amino-5-bromophenyl)-2-hydroxy-6-oxhexa-2,4-dienoic via gas chromatograph-mass spectrometry (GC-MS) analysis. The metabolite disappeared after 14 d, suggesting that the metabolite can also be degraded by strain CH-1. 3-BCZ is a new persistent organic pollutant. This is the first report of the biodegradation of 3-BCZ. The results indicated that strain CH-1 may be a promising bacterial candidate for the bioremediation of environments polluted with polyhalogenated carbazoles (PHCs).
在本研究中，从受污染土壤中分离出一株能够降解3-溴咔唑（3-BCZ）的细菌菌株CH-1。基于其生理生化特性和16S rRNA基因，菌株CH-1被鉴定为嗜麦芽窄食单胞菌属。在pH 7.0和30℃的矿物盐培养基（MSM）中，菌株CH-1能够在8天内降解50 mg/L的3-BCZ中的70%。在此过程中，通过气相色谱-质谱联用（GC-MS）分析，主要中间代谢产物被鉴定为（2E，4Z）-6-（2-氨基-5-溴苯基）-2-羟基-6-氧代-2，4-己二烯酸。14天后该代谢产物消失，表明该代谢产物也可被菌株CH-1降解。3-BCZ是一种新型持久性有机污染物。这是关于3-BCZ生物降解的首次报道。结果表明，菌株CH-1可能是用于生物修复多卤代咔唑（PHCs）污染环境的有前景的细菌候选菌株。
