Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, Laboratory of Insect Toxicology, South China Agricultural University, Guangzhou 510642, People's Republic of China.
Appl Microbiol Biotechnol. 2011 Apr;90(2):755-67. doi: 10.1007/s00253-010-3035-z. Epub 2010 Dec 24.
A bacterial strain ZS-S-01, newly isolated from activated sludge, could effectively degrade fenvalerate and its hydrolysis product 3-phenoxybenzoic acid (3-PBA). Based on the morphology, physiological biochemical characteristics, and 16 S rDNA sequence, strain ZS-S-01 was identified as Stenotrophomonas sp. Strain ZS-S-01 could also degrade and utilize deltamethrin, beta-cypermethrin, beta-cyfluthrin, and cyhalothrin as substrates for growth. Strain ZS-S-01 was capable of degrading fenvalerate rapidly without a lag phase over a wide range of pH and temperature, even in the presence of other carbon sources, and metabolized it to yield 3-PBA, then completely degraded it. No persistent accumulative product was detected by HPLC and GC/MS analysis. Studies on biodegradation in various soils showed that strain ZS-S-01 demonstrated efficient degradation of fenvalerate and 3-PBA (both 50 mg·kg(-1)) with a rate constant of 0.1418-0.3073 d(-1), and half-lives ranged from 2.3 to 4.9 days. Compared with the controls, the half-lives for fenvalerate and 3-PBA reduced by 16.9-156.3 days. These results highlight strain ZS-S-01 may have potential for use in bioremediation of pyrethroid-contaminated environment.
一株从活性污泥中分离得到的细菌 ZS-S-01 能有效降解氰戊菊酯及其水解产物 3-苯氧苯甲酸（3-PBA）。根据形态、生理生化特性和 16S rDNA 序列，该菌株被鉴定为 Stenotrophomonas sp.。ZS-S-01 菌株还能以氯氰菊酯、β-氯氰菊酯、β-氟氯氰菊酯和氰戊菊酯为生长基质进行降解和利用。ZS-S-01 菌株在较宽的 pH 和温度范围内能迅速降解氰戊菊酯而无需适应期，即使存在其他碳源，也能将其代谢为 3-PBA，然后完全降解。HPLC 和 GC/MS 分析未检测到持久性累积产物。在各种土壤中的生物降解研究表明，ZS-S-01 菌株能有效降解氰戊菊酯和 3-PBA（均为 50mg·kg(-1))，速率常数为 0.1418-0.3073d(-1)，半衰期范围为 2.3-4.9 天。与对照相比，氰戊菊酯和 3-PBA 的半衰期缩短了 16.9-156.3 天。这些结果表明，ZS-S-01 菌株可能具有用于修复拟除虫菊酯污染环境的潜力。
