在发酵培养基中加入生物炭提高产纤维梭菌利用合成气生产乙醇的能力。
Enhanced ethanol production by Clostridium ragsdalei from syngas by incorporating biochar in the fermentation medium.
机构信息
Department of Biosystems and Agricultural Engineering, Oklahoma State University, Stillwater, OK, USA.
Department of Biosystems and Agricultural Engineering, Oklahoma State University, Stillwater, OK, USA.
出版信息
Bioresour Technol. 2018 Jan;247:291-301. doi: 10.1016/j.biortech.2017.09.060. Epub 2017 Sep 9.
Biochar contains minerals and metals that can serve as nutrients for acetogens to produce ethanol via syngas fermentation. In this study, four fermentation media containing biochar from switchgrass (SGBC), forage sorghum (FSBC), red cedar (RCBC) and poultry litter (PLBC) were compared with standard yeast extract (YE) medium for syngas fermentation using Clostridium ragsdalei. Fermentations were performed in 250mL bottle reactors at 150rpm and 37°C with syngas containing CO:H:CO (40:30:30) by volume. Results showed that media containing RCBC and PLBC improved ethanol production by 16.3% and 58.9%, respectively, compared to YE medium. C. ragsdalei consumed 69% more H and 40% more CO in PLBC medium compared to YE medium. However, no enhancement of ethanol production was observed in SGBC and FSBC media. The highest release of Na, K, Ca, Mg, S and P was from PLBC, which was considered to contribute in enhancement of ethanol production.
生物炭含有矿物质和金属，可作为产乙酸菌的营养物质，通过合成气发酵生产乙醇。在这项研究中，使用产绒革脂菌（Clostridium ragsdalei）比较了四种发酵培养基，分别是来自柳枝稷的生物炭（SGBC）、饲用高粱的生物炭（FSBC）、红雪松的生物炭（RCBC）和家禽粪肥的生物炭（PLBC），以及标准的酵母提取物（YE）培养基用于合成气发酵。发酵在 250mL 瓶式反应器中进行，转速为 150rpm，温度为 37°C，用体积比为 40:30:30 的 CO:H:CO 合成气。结果表明，与 YE 培养基相比，含有 RCBC 和 PLBC 的培养基分别将乙醇产量提高了 16.3%和 58.9%。与 YE 培养基相比，产绒革脂菌在 PLBC 培养基中消耗了 69%更多的 H 和 40%更多的 CO。然而，在 SGBC 和 FSBC 培养基中没有观察到乙醇产量的提高。PLBC 中释放出的 Na、K、Ca、Mg、S 和 P 最多，这被认为有助于提高乙醇产量。
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