Department of Biosystems and Agricultural Engineering, Oklahoma State University, Stillwater, OK 74078, USA.
Department of Biosystems and Agricultural Engineering, Oklahoma State University, Stillwater, OK 74078, USA.
Bioresour Technol. 2013 Nov;147:508-515. doi: 10.1016/j.biortech.2013.08.075. Epub 2013 Aug 22.
The development of a low cost medium for ethanol production is critical for process feasibility. Ten media were formulated for Clostridium ragsdalei by reduction, elimination and replacement of expensive nutrients. Cost analysis and effects of medium components on growth and product formation were investigated. Fermentations were performed in 250 mL bottles using syngas (20% CO, 15% CO2, 5% H2 and 60% N2). The standard medium M1 cost is $9.83/L, of which 93% is attributed to morpholinoethane sulfonic acid (MES) buffer. Statistical analysis of the results showed that MES removal did not affect cell growth and ethanol production (P>0.05). Based on cells' elemental composition, a minimal mineral concentration medium M7 was formulated, which provided 29% higher ethanol yield from CO at 3% of the cost compared to medium M1. Ethanol yield from CO in the completely defined medium M9 was 36% higher than while at 5% the cost of medium M1.
开发低成本的乙醇生产培养基对于工艺可行性至关重要。通过降低、消除和替代昂贵的营养物质，为 Clostridium ragsdalei 设计了十种培养基。对培养基成分的成本分析以及对生长和产物形成的影响进行了研究。发酵在 250 mL 瓶中进行，使用合成气（20% CO、15% CO2、5% H2 和 60% N2）。标准培养基 M1 的成本为 9.83 美元/升，其中 93%归因于吗啉代乙磺酸（MES）缓冲液。结果的统计分析表明，去除 MES 不会影响细胞生长和乙醇生产（P>0.05）。根据细胞的元素组成，设计了一种最小矿物浓度培养基 M7，与培养基 M1 相比，从 CO 中获得的乙醇产率提高了 29%，成本仅为培养基 M1 的 3%。在完全定义的培养基 M9 中，CO 的乙醇产率比培养基 M1 的成本高 5%时提高了 36%。
