Glicksman M A, Brower D L
Department of Molecular and Cellular Biology, University of Arizona, Tucson 85721.
Dev Biol. 1988 Apr;126(2):219-27. doi: 10.1016/0012-1606(88)90132-7.
Using monoclonal antibodies specific for their protein products, the expression of the Ubx, Antp, and Scr genes was examined in imaginal discs and central nervous systems of esc-Drosophila larvae. In esc-mutants, both the Ubx and Scr proteins are expressed at increased levels or in new locations in the leg discs. Ubx also is expressed in new locations in the posterior wing disc and in small groups of cells in the antenna disc. The Antp protein is expressed ectopically in the eye-antenna disc; however, obvious abnormal expression of Antp was not found in the thoracic imaginal discs. Particularly striking is the fact that a single disc, such as the mesothoracic leg, can show increased expression of both a more "anterior" homeotic gene (Scr) and a more "posterior" gene (Ubx). Ectopic expression of Ubx and Antp, but not of Scr, is seen in the central nervous system of mutant larvae. These results are discussed with respect to the adult esc-phenotype and the differential effects of esc mutations on early and late development.
利用针对其蛋白质产物的单克隆抗体，在esc - 果蝇幼虫的成虫盘和中枢神经系统中检测了Ubx、Antp和Scr基因的表达。在esc突变体中，Ubx和Scr蛋白在腿盘中均以增加的水平表达或在新的位置表达。Ubx也在后翅盘中的新位置以及触角盘中的小细胞群中表达。Antp蛋白在眼 - 触角盘中异位表达；然而，在胸成虫盘中未发现Antp明显的异常表达。特别引人注目的是，单个盘，如中胸腿盘，可以同时显示出一个更“前部”的同源异型基因（Scr）和一个更“后部”的基因（Ubx）的表达增加。在突变幼虫的中枢神经系统中可见Ubx和Antp的异位表达，但Scr没有。针对成虫esc表型以及esc突变对早期和晚期发育的不同影响对这些结果进行了讨论。
