中国南方急性新生儿细菌性结膜炎细菌病原体变化的时序性研究。

A chronological study of the bacterial pathogen changes in acute neonatal bacterial conjunctivitis in southern China.

作者信息

Tang Song, Li Ming, Chen Hongbo, Ping Guo, Zhang Chun, Wang Shusheng

机构信息

Shenzhen Eye Hospital, Key laboratory of Ophthalmology, Affiliated Shenzhen Eye Hospital of Ji-nan University, Shenzhen, 518000, People's Republic of China.

Department of Cell and Molecular Biology, Tulane University, 2000 Percival Stern Hall, 6400 Freret Street, New Orleans, LA, 70118, USA.

出版信息

BMC Ophthalmol. 2017 Sep 26;17(1):174. doi: 10.1186/s12886-017-0570-8.

DOI:10.1186/s12886-017-0570-8

PMID:28950852

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5615761/

Abstract

BACKGROUND

The aim of the project is to retrospectively study the changes in bacterial pathogens in acute neonatal bacterial conjunctivitis from 2002 to 2016 in Southern China. The results may provide the guidance for drug choice for acute neonatal bacterial conjunctivitis.

METHODS

Secretion specimens for bacterial culture were taken from 485 cases with clinically diagnosed acute bacterial neonatal conjunctivitis. Bacterial pathogens were detected by Gram staining and subsequent bacterial culture.

RESULTS

From the analysis of the bacterial pathogens in 485 cases of acute neonatal conjunctivitis patients from 2002 to 2016 in Southern China, there is an overall trend of decreasing detection of Gram-positive bacteria and increasing detection of Gram-negative bacteria from the conjunctival sac secretions. Gram-positive bacteria in the bacteria-positive samples dropped year by year from 82.6% in 2002 to 72.4% in 2016. Accordingly, the ratio of Gram-negative bacteria increased from 17.4% in 2002 to 27.6% in 2016. Of note, despite the overall trend, there was a significant increase in detection of Gram-positive bacteria and decrease in detection of Gram-negative bacteria from 2011 to 2012. Among the Gram-positive bacteria, there is a trend of increasing percentage of the opportunistic pathogens (an ~~60% increase in Staphylococcus epidermidis and Staphylococcus saprophytic) and decreasing percentage of Staphylococcus aureus (~~30% decrease) and hemolytic streptococcus (~20% decrease) in the last 15 years. The main Gram-negative bacterium is Neisseria gonorrhoeae. Overall, there is a change in the pattern of bacterial species in acute neonatal bacterial conjunctivitis in Southern China in the last 15 years.

CONCLUSION

Our study provides a trend analysis of the bacterial pathogens in the conjunctival sac secretions of the acute neonatal bacterial conjunctivitis patients in Southern China in recent years. This data could provide useful information regarding the treatment options for neonatal bacterial conjunctivitis.

摘要

背景

本项目旨在回顾性研究2002年至2016年中国南方急性新生儿细菌性结膜炎中细菌病原体的变化情况。研究结果可为急性新生儿细菌性结膜炎的药物选择提供指导。

方法

从485例临床诊断为急性细菌性新生儿结膜炎的患者中采集用于细菌培养的分泌物标本。通过革兰氏染色及后续细菌培养检测细菌病原体。

结果

对2002年至2016年中国南方485例急性新生儿结膜炎患者的细菌病原体分析显示，结膜囊分泌物中革兰氏阳性菌的检出率总体呈下降趋势，革兰氏阴性菌的检出率呈上升趋势。细菌阳性样本中革兰氏阳性菌从2002年的82.6%逐年降至2016年的72.4%。相应地，革兰氏阴性菌的比例从2002年的17.4%增至2016年的27.6%。值得注意的是，尽管总体呈上述趋势，但在2011年至2012年期间革兰氏阳性菌的检出率显著上升，革兰氏阴性菌的检出率下降。在革兰氏阳性菌中，过去15年机会性病原体的百分比呈上升趋势（表皮葡萄球菌和腐生葡萄球菌增加约60%），金黄色葡萄球菌（下降约30%）和溶血性链球菌（下降约20%）的百分比呈下降趋势。主要革兰氏阴性菌为淋病奈瑟菌。总体而言，过去15年中国南方急性新生儿细菌性结膜炎的细菌种类模式发生了变化。