Department of Laboratory Medicine, Hangzhou First People's Hospital, Hangzhou, Zhejiang 310006, China.
Hangzhou Cancer Institution, Hangzhou Cancer Hospital, Hangzhou, Zhejiang 310002, China.
Dis Markers. 2017;2017:2929381. doi: 10.1155/2017/2929381. Epub 2017 Aug 29.
This study sought to evaluate the diagnostic value of the methylation of multiple gene promoters in serum in hepatitis B virus- (HBV-) related hepatocellular carcinoma (HCC). A total of 343 participants were enrolled, including 98 patients with HCC, 75 patients with liver cirrhosis (LC), 90 patients with chronic hepatitis B (CHB), and 80 healthy individuals. , , , , , and were selected as the candidate genes. The MethyLight method was used to assay promoter methylation statuses. The diagnostic performances of markers were assessed by constructing receiver operating characteristic (ROC) curves. The prevalences of methylation for , , , , , and were 52.04%, 36.73%, 29.59%, 20.41%, 17.35%, and 11.22%, respectively. methylation completely overlapped with methylation. The area under the curve (AUC) for methylation (0.718) was better than the corresponding AUC for AFP (0.609) in distinguishing HCC from CHB. When , , , and AFP were combined, the AUC was 0.852 (95% CI = 0.796-0.908, = 0.028), and the sensitivity and specificity were 83.7% and 78.9%, respectively. In conclusion, an assay that combines methylation of the , , and gene promoters in serum and AFP could significantly improve HBV-related HCC diagnoses.
本研究旨在评估血清中多个基因启动子甲基化在乙型肝炎病毒（HBV）相关肝细胞癌（HCC）中的诊断价值。共纳入 343 名参与者，包括 98 例 HCC 患者、75 例肝硬化（LC）患者、90 例慢性乙型肝炎（CHB）患者和 80 名健康个体。选择、、、、和作为候选基因。采用 MethyLight 方法检测启动子甲基化状态。通过构建受试者工作特征（ROC）曲线评估标志物的诊断性能。甲基化的患病率分别为、、、、和，分别为 52.04%、36.73%、29.59%、20.41%、17.35%和 11.22%。甲基化完全与甲基化重叠。与 AFP 相比，甲基化的曲线下面积（AUC）（0.718）在区分 HCC 与 CHB 方面更好。当、、和 AFP 结合时，AUC 为 0.852（95%CI=0.796-0.908，=0.028），灵敏度和特异性分别为 83.7%和 78.9%。总之，联合检测血清中、、和基因启动子甲基化与 AFP 可显著提高 HBV 相关 HCC 的诊断。
