Frank R S, Moursi M M, Podrazik R M, Zelenock G B, D'Alecy L G
Department of Physiology, University of Michigan Medical School, Ann Arbor 48109.
J Vasc Surg. 1988 Apr;7(4):515-23.
To better understand renal and systemic hemodynamics associated with hindquarter ischemia produced by aortic compression, chloralose-anesthetized dogs were given phentolamine while an external clamp maintained infrarenal aortic pressure below 25 mm Hg for 45 minutes. In four sham-operated dogs, infrarenal pressure was maintained; reinforced cannulas, capable of resisting clamp compression, were placed within the aorta and the inferior vena cava. Suprarenal and infrarenal arterial pressure and renal blood flow were continuously monitored. Blood samples taken before clamp application and at 1, 3, 5, and 10 minutes after clamp removal were assayed for adenosine, inosine, xanthine, and hypoxanthine. On clamp removal suprarenal pressure immediately dropped from a preclamp pressure of 114 to 82 mm Hg but returned to preclamp values within 1 minute. Renal blood flow was significantly reduced after clamp release, reaching a nadir of 39% of preclamp flow. This reduction persisted despite a normalization of arterial pressure. Summed plasma purines were significantly elevated 1 minute after clamp removal. Sham-operated dogs showed no significant alterations in arterial pressure, renal blood flow, or plasma purine levels. This study demonstrates a significant non-alpha-adrenergic receptor-mediated reduction in renal blood flow and a coincident increase in purine degradation products after removal of an infrarenal aortic cross-clamp.
为了更好地理解与主动脉压迫导致的后肢缺血相关的肾脏和全身血流动力学，对用氯醛糖麻醉的狗给予酚妥拉明，同时用外部夹子将肾下主动脉压力维持在25 mmHg以下45分钟。在四只假手术狗中，维持肾下压力；将能够抵抗夹子压迫的加强套管置于主动脉和下腔静脉内。连续监测肾上腺和肾下动脉压力以及肾血流量。在夹闭前以及夹闭解除后1、3、5和10分钟采集的血样检测腺苷、肌苷、黄嘌呤和次黄嘌呤。夹闭解除后，肾上腺压力立即从夹闭前的114 mmHg降至82 mmHg，但在1分钟内恢复到夹闭前的值。夹闭解除后肾血流量显著减少，降至夹闭前血流量的39%的最低点。尽管动脉压恢复正常，但这种减少仍持续存在。夹闭解除后1分钟，血浆嘌呤总和显著升高。假手术狗的动脉压、肾血流量或血浆嘌呤水平无显著变化。本研究表明，肾下主动脉夹闭解除后，存在显著的非α-肾上腺素能受体介导的肾血流量减少以及嘌呤降解产物同时增加。
