Hadadianpour Azadeh, Samiee Aref Mohammad Hasan, Zeinali Sirous
Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran.
Kawsar Human Genetics Research Center, Tehran, Iran.
Iran Biomed J. 2018 Mar;22(2):134-7. doi: 10.22034/ibj.22.2.134. Epub 2017 Sep 27.
Human leukocyte antigen (HLA) gene is a highly polymorphic region. HLA typing is required to match patients and donors for transplantation; therefore, development of HLA registries is necessary for finding HLA match donors. HLA system is highly informative, and numerous studies have been conducted on HLA allele distribution in different populations.
In this study, 100 unrelated Iranian individuals were typed for HLA-A locus using sequence-based typing method. Samples were subjected to the PCR, followed by Sanger sequencing and software analysis.
A02:01 (13%) and A24:02 (12%) were the two most frequent alleles, while A01:14, A02:05, A02:11, A02:34, A02:50, A11:04, A23:02, A24:34, A25:01, A26:09, A26:43, A29:67, A30:54, A31:02, A31:66, A32:03, A32:04, A33:03, and A*66:15 alleles had the least frequencies (1%).
This is the first report of HLA-A allele level typing in a randomized population of Iran and can be useful for development of national registries of HLA-typed volunteer marrow donors and local cord blood banks.
人类白细胞抗原（HLA）基因是一个高度多态性的区域。移植时需要进行HLA分型以匹配患者和供体；因此，建立HLA登记库对于寻找HLA匹配的供体是必要的。HLA系统信息量大，并且已经针对不同人群中的HLA等位基因分布进行了大量研究。
在本研究中，使用基于序列的分型方法对100名无亲缘关系的伊朗个体进行了HLA-A位点分型。样本先进行PCR，然后进行桑格测序和软件分析。
A02:01（13%）和A24:02（12%）是两个最常见的等位基因，而A01:14、A02:05、A02:11、A02:34、A02:50、A11:04、A23:02、A24:34、A25:01、A26:09、A26:43、A29:67、A30:54、A31:02、A31:66、A32:03、A32:04、A33:03和A*66:15等位基因的频率最低（1%）。
这是伊朗随机人群中HLA-A等位基因水平分型的首次报告，可用于建立HLA分型的志愿骨髓供体国家登记库和当地脐带血库。
