Leong Wai Yean, Soon Chin Fhong, Wong Soon Chuan, Tee Kian Sek, Cheong Sok Ching, Gan Siew Hua, Youseffi Mansour
Faculty of Electrical and Electronic Engineering, Universiti Tun Hussein Onn Malaysia, 86400 Parit Raja, Batu Pahat, Johor, Malaysia.
Biosensor and Bioengineering Laboratory, MiNT-SRC Research Center, Universiti Tun Hussein Onn Malaysia, 86400 Parit Raja, Batu Pahat, Johor, Malaysia.
Bioengineering (Basel). 2017 May 14;4(2):43. doi: 10.3390/bioengineering4020043.
Cells encapsulation is a micro-technology widely applied in cell and tissue research, tissue transplantation, and regenerative medicine. In this paper, we proposed a growth of microtissue model for the human keratinocytes (HaCaT) cell line and an oral squamous cell carcinoma (OSCC) cell line (ORL-48) based on a simple aerosol microencapsulation technique. At an extrusion rate of 20 μL/min and air flow rate of 0.3 L/min programmed in the aerosol system, HaCaT and ORL-48 cells in alginate microcapsules were encapsulated in microcapsules with a diameter ranging from 200 to 300 μm. Both cell lines were successfully grown into microtissues in the microcapsules of alginate within 16 days of culture. The microtissues were characterized by using a live/dead cell viability assay, field emission-scanning electron microscopy (FE-SEM), fluorescence staining, and cell re-plating experiments. The microtissues of both cell types were viable after being extracted from the alginate membrane using alginate lyase. However, the microtissues of HaCaT and ORL-48 demonstrated differences in both nucleus size and morphology. The microtissues with re-associated cells in spheroids are potentially useful as a cell model for pharmacological studies.
细胞封装是一种广泛应用于细胞和组织研究、组织移植及再生医学的微技术。在本文中，我们基于一种简单的气溶胶微封装技术，提出了一种针对人角质形成细胞（HaCaT）细胞系和口腔鳞状细胞癌（OSCC）细胞系（ORL - 48）的微组织生长模型。在气溶胶系统中设定的挤出速率为20 μL/分钟和气流速率为0.3 L/分钟的条件下，藻酸盐微胶囊中的HaCaT和ORL - 48细胞被封装在直径为200至300 μm的微胶囊中。在培养16天内，两种细胞系均成功在藻酸盐微胶囊中生长为微组织。通过活/死细胞活力测定、场发射扫描电子显微镜（FE - SEM）、荧光染色和细胞再接种实验对微组织进行了表征。使用藻酸酶从藻酸盐膜中提取后，两种细胞类型的微组织均具有活力。然而，HaCaT和ORL - 48的微组织在细胞核大小和形态上均表现出差异。球体中重新聚集细胞的微组织可能作为药理学研究的细胞模型。
