利用CRISPR/Cas9技术生成ASGR1纯合突变人类胚胎干细胞系WAe001-A-6
Generation of an ASGR1 homozygous mutant human embryonic stem cell line WAe001-A-6 using CRISPR/Cas9.
作者信息
Xu Yingying, Wu Yuhang, Guo Dongsheng, Gao Ge, Lai Keyu, Yang Fan, Wang Kepin, Wu Han, Lai Liangxue, Li Jialiang, Xu Kecheng, Li Yin-Xiong
机构信息
Institute of Public Health, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China; Guangdong Provincial Key Laboratory of Biocomputing, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China; Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China; University of Chinese Academy of Sciences, Beijing, China.
Institute of Public Health, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China; Guangdong Provincial Key Laboratory of Biocomputing, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China; Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China.
出版信息
Stem Cell Res. 2017 Jul;22:29-32. doi: 10.1016/j.scr.2017.05.006. Epub 2017 May 19.
The gene asialoglycoprotein receptor 1 (ASGR1) encodes a subunit of the asialoglycoprotein receptor. Here we report the generation of a human embryonic stem cell line WAe001-A-6 harbouring homozygous ASGR1 mutations using CRISPR/Cas9. The mutation involves a 37bp deletion, resulting in a frame shift. The homozygous knockout WA01 cell line maintains a normal karyotype, typical stem cell morphology, pluripotency and differentiation potential in vitro.
去唾液酸糖蛋白受体1（ASGR1）基因编码去唾液酸糖蛋白受体的一个亚基。在此，我们报告利用CRISPR/Cas9技术构建出携带纯合ASGR1突变的人胚胎干细胞系WAe001-A-6。该突变涉及37bp的缺失，导致移码突变。纯合敲除的WA01细胞系在体外维持正常核型、典型的干细胞形态、多能性及分化潜能。
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