IEEE Trans Biomed Circuits Syst. 2017 Dec;11(6):1438-1449. doi: 10.1109/TBCAS.2017.2748158. Epub 2017 Sep 22.
A dual-channel credit-card-sized impedance cell counter featuring a throughput of 2000 cell/s and detection of single yeast cells (5 μm) with a signal-to-noise ratio of 20 dB is presented. Its compactness is achieved by a CMOS ASIC combining a lock-in impedance demodulator with an oversampling 20-bit ΣΔ ADC and real-time peak detection embedded in field-programmable gate array. The module is coupled to a dielectrophoretic cell-sorting microfluidic device, offering compact and label-free electrical readout that replaces the need for a fluorescence microscope and, thus, is suitable for point-of-care diagnostics. The independent role of each dimension of the planar sensing microelectrodes is demonstrated, with simulations and experiments, along with its relevant effect on the spectrum of thin channels, deriving useful design guidelines.
本文介绍了一种双通道信用卡大小的阻抗细胞计数器，其吞吐量为 2000 个细胞/s，能够检测到信噪比为 20dB 的单个酵母细胞（5μm）。该计数器的紧凑性得益于一种 CMOS ASIC，该 ASIC 将锁相阻抗解调器与过采样 20 位ΣΔ ADC 以及现场可编程门阵列中的实时峰值检测相结合。该模块与介电泳细胞分选微流控装置相耦合，提供了紧凑的、无需标记的电读出功能，取代了荧光显微镜的需求，因此适用于即时诊断。通过模拟和实验，证明了平面感应微电极的每个尺寸的独立作用及其对薄通道谱的相关影响，从而得出了有用的设计准则。
