基于多孔石墨烯的双生物催化信号放大葡萄糖生物传感器
Double Biocatalysis Signal Amplification Glucose Biosensor Based on Porous Graphene.
作者信息
He Yaping, Zheng Jianbin, Wang Bini, Ren Hongjiang
机构信息
School of Chemical Engineering, Xi'an University, Xi'an 710065, Shaanxi, China.
Institute of Analytical Science/Shaanxi Provincial Key Laboratory of Electroanalytical Chemistry, Northwest University, Xi'an 710069, Shaanxi, China.
出版信息
Materials (Basel). 2017 Sep 27;10(10):1139. doi: 10.3390/ma10101139.
Controllable preparation of nanopores to promote the performance of electrochemical biosensing interfaces has become one of the researching frontiers in biosensing. A double biocatalysis signal amplification of glucose biosensor for the study of electrochemical behaviors of glucose oxidase (GOx) was proposed by using horseradish peroxidase biosynthesized porous graphene (PGR) as the platform for the biocatalytic deposition of gold nanoparticles (AuNPs). The biosensor showed a linear range from 0.25 to 27.5 μM with a detection limit of 0.05 μM (S/N = 3) towards glucose. Furthermore, the proposed AuNPs/GOx-PGR modified glassy carbon electrode (AuNPs/GOx-PGR/GCE) achieved direct electron transfer of GOx.
可控制备纳米孔以提升电化学生物传感界面的性能已成为生物传感领域的研究前沿之一。以辣根过氧化物酶生物合成的多孔石墨烯（PGR）为平台进行金纳米颗粒（AuNPs）的生物催化沉积，提出了一种用于研究葡萄糖氧化酶（GOx）电化学行为的葡萄糖生物传感器双生物催化信号放大方法。该生物传感器对葡萄糖的线性范围为0.25至27.5 μM，检测限为0.05 μM（S/N = 3）。此外，所提出的AuNPs/GOx-PGR修饰玻碳电极（AuNPs/GOx-PGR/GCE）实现了GOx的直接电子转移。
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