Loh Yean Chun, Tan Chu Shan, Ch'ng Yung Sing, Ahmad Mariam, Ng Chiew Hoong, Yam Mun Fei
1 Department of Pharmacology, School of Pharmaceutical Sciences, Universiti Sains Malaysia , Minden, Penang, Malaysia .
2 College of Pharmacy, Fujian University of Traditional Chinese Medicine , Fuzhou, Fujian, China .
J Med Food. 2017 Dec;20(12):1201-1213. doi: 10.1089/jmf.2017.3958. Epub 2017 Sep 27.
Hypertension, one of the famous "silent killers" that can attack people at any age, is a current hot topic among scientists due to multiple syndromic behavior and concomitant diseases. The new scientific-based Traditional Chinese Medicine (TCM) formulation approach was used in a previous study by combining five TCM herbs, including Gastrodia elata Bl., Uncaria rhynchophylla (Miq.) Miq. ex Havil., Pueraria thomsonii Benth., Panax notoginseng (Burk.) F.H. Chen, and Alisma orientalis (Sam.) Juzep in optimized ratio (named BPAid). The objective of the present study was to evaluate the mechanism pathways employed by BPAid for vasodilatory effect with the use of an in vitro isolated aortic rings assay. Interestingly, all the mechanisms investigated were involved in the BPAid's vasodilation activity in which the majority contributed through the nitric oxide/soluble guanylyl cyclase/cyclic guanosine monophosphate (NO/sGC/cGMP) pathways, followed by prostacyclin (PGI, β-adrenergic, and M-receptors pathways. Furthermore, the BPAid appeared to manage vascular tone by regulating action potential through potassium and both voltage-operated calcium channel and inositol triphosphate receptor (IPR) pathways. The results obtained has confirmed the expected outcome that the benefits of TCM herbs in BPAid can meet the criteria of counteracting multiple signaling mechanism pathways involved in the etiology of hypertension. In addition to this study, the fingerprints and chemical properties of BPAid was identified by using tri-step Fourier transform infrared spectroscopy and compared with its derivatives. The results obtained suggested that the majority of the vasodilatory effects exerted by BPAid were attributed to the presence of saponins and aromatic ring-containing vasoactive compounds.
高血压是著名的“沉默杀手”之一，可在任何年龄段侵袭人群，由于其多种综合征行为和伴随疾病，它是当前科学家们研究的热点话题。在之前的一项研究中，采用了基于科学的新型中药配方方法，将五种中药按优化比例组合，包括天麻、钩藤、葛根、三七和泽泻（命名为BPAid）。本研究的目的是通过体外分离主动脉环试验评估BPAid产生血管舒张作用的机制途径。有趣的是，所有研究的机制都参与了BPAid的血管舒张活性，其中大部分是通过一氧化氮/可溶性鸟苷酸环化酶/环磷酸鸟苷（NO/sGC/cGMP）途径发挥作用，其次是前列环素（PGI）、β-肾上腺素能和M受体途径。此外，BPAid似乎通过钾离子、电压门控钙通道和肌醇三磷酸受体（IPR）途径调节动作电位来控制血管张力。获得的结果证实了预期的结果，即BPAid中中药的益处可以满足对抗高血压病因中涉及的多种信号机制途径这一标准。除了本研究外，还通过三步傅里叶变换红外光谱法鉴定了BPAid的指纹图谱和化学性质，并与其衍生物进行了比较。获得的结果表明，BPAid发挥的大部分血管舒张作用归因于皂苷和含芳香环的血管活性化合物的存在。
