Loh Yean Chun, Tan Chu Shan, Ch'ng Yung Sing, Ahmad Mariam, Asmawi Mohd Zaini, Yam Mun Fei
1 Department of Pharmacology, School of Pharmaceutical Sciences, Universiti Sains Malaysia , Minden, Malaysia .
2 College of Pharmacy, Fujian University of Traditional Chinese Medicine , Fuzhou, China .
J Med Food. 2017 Mar;20(3):265-278. doi: 10.1089/jmf.2016.3836.
Recently, a new syndromic disease combination theory of traditional Chinese medicine (TCM) for hypertensive treatment has been introduced. In the wake of this new concept, a new science-based TCM formula that counteracts various syndromes is needed. The objective of this study was to develop such a formula. Five of the most clinically prescribed TCM herbs that work on different syndromes, namely Gastrodia elata, Uncaria rhynchophylla, Pueraria thomsonii, Panax notoginseng, and Alisma orientale, were selected for this study. The fingerprints of these five herbs were analyzed by tri-step Fourier transform infrared spectroscopy. Three different solvents, 95% ethanol, 50% ethanol, and distilled water, were used for the maceration of the herbs and their vasodilatory effects were studied using in vitro precontracted aortic ring model. Among these, the 50% ethanolic extracts of G. elata (GE50) and A. orientale (AO50), and 95% ethanolic extracts of U. rhynchophylla (UR95), P. thomsonii (PT95), and P. notoginseng (PN95) were found to be the most effective for eliciting vasodilation. Thus, these five extracts were used for orthogonal stimulus-response compatibility group studies by using L (5) formula. The best combination ratio for GE50, UR95, PT95, PN95, and AO50, which was assigned as Formula 1 (F1), was found at EC, EC, EC, EC, and EC, respectively. The vasodilatory effect of the extracts prepared from different extraction methods using F1 ratio was also studied. From the results, the EC and R of total 50% ethanolic extract of five herbs using F1 ratio (F1-2) were 0.028 ± 0.005 mg/mL and 101.71% ± 3.64%, with better values than F1 (0.104 ± 0.014 mg/mL and 97.80% ± 3.12%, respectively). In conclusion, the optimum ratio and appropriate extraction method (F1-2) for the new TCM formula were revealed.
最近，一种用于高血压治疗的中医新综合征疾病组合理论被提出。在此新概念之后，需要一种基于科学的新的中医方剂来对抗各种综合征。本研究的目的就是开发这样一种方剂。本研究选取了临床上最常用的针对不同综合征的五种中药，即天麻、钩藤、葛根、三七和泽泻。采用三步傅里叶变换红外光谱法分析这五种中药的指纹图谱。使用三种不同的溶剂，即95%乙醇、50%乙醇和蒸馏水对中药进行浸渍，并使用体外预收缩主动脉环模型研究它们的血管舒张作用。其中，天麻50%乙醇提取物（GE50）和泽泻50%乙醇提取物（AO50），以及钩藤95%乙醇提取物（UR95）、葛根95%乙醇提取物（PT95）和三七95%乙醇提取物（PN95）被发现对引起血管舒张最有效。因此，使用这五种提取物通过L(5)公式进行正交刺激-反应配伍组研究。分别在EC、EC、EC、EC和EC时发现了GE50、UR95、PT95、PN95和AO50的最佳组合比例，该组合被指定为方剂1（F1）。还研究了使用F1比例从不同提取方法制备的提取物的血管舒张作用。结果表明，使用F1比例的五种中药总50%乙醇提取物（F1-2）的EC和R分别为0.028±0.005mg/mL和101.71%±3.64%，比F1（分别为0.104±0.014mg/mL和97.80%±3.12%）的值更好。总之，揭示了新中医方剂的最佳比例和合适的提取方法（F1-2）。
