Jamil Zehra, Perveen Khalida, Malik Rabia, Avesi Lubna
Aga Khan University.
Dow University of Health Sciences, Karachi.
J Pak Med Assoc. 2017 Oct;67(10):1470-1475.
To evaluate the strength of anti-mullerian hormone in reflecting the stages of ovarian toxicity-induced by cyclophosphamide.
This study was conducted in December 2014 and comprised female mice that were divided into four groups: group A served as control, group B received three weekly injections of cyclophosphamide, group C was co administered alpha-tocopherol along with cyclophosphamide, while group D solely received alpha-tocopherol. The ovaries were evaluated for follicular dynamics, and anti-mullerian hormone was assessed using mouse enzyme-linked immunosorbent assay kit. The data was analysed using SPSS 19.
There were 40 mice in the study. Histological analysis revealed severely reduced ovarian reserve in group B(p<0.01).In group C alpha-tocopherol conserved the ovarian reserve to near normal, thus follicle count was significantly higher than group B (p<0.05). However, this moderate reduction was still lower than the controls (p<0.01). Furthermore, the number of corpus lutea and atretic follicles were significantly higher in groups B and C (p<0.01). Regarding hormonal analyses in comparison to controls, anti-mullerian hormone levels were low in group B (p<0.01), while group C reported an insignificant fall in serum anti-mullerian hormone levels (p=0.101).
There was substantial evidence that anti-mullerian hormone monitoring during chemotherapy administration may fulfil the criteria of earliest diagnostic indicator of secondary infertility.
评估抗苗勒管激素在反映环磷酰胺诱导的卵巢毒性阶段方面的作用强度。
本研究于2014年12月进行，选用雌性小鼠并分为四组：A组作为对照组，B组每周注射三次环磷酰胺，C组在注射环磷酰胺的同时联合给予α-生育酚，D组仅给予α-生育酚。评估卵巢的卵泡动态变化，并使用小鼠酶联免疫吸附测定试剂盒检测抗苗勒管激素。数据采用SPSS 19进行分析。
本研究共有40只小鼠。组织学分析显示，B组卵巢储备严重减少（p<0.01）。在C组中，α-生育酚使卵巢储备维持在接近正常水平，因此卵泡计数显著高于B组（p<0.05）。然而，这种中度减少仍低于对照组（p<0.01）。此外，B组和C组的黄体和闭锁卵泡数量显著增加（p<0.01）。与对照组相比，激素分析结果显示，B组抗苗勒管激素水平较低（p<0.01），而C组血清抗苗勒管激素水平下降不显著（p=0.101）。
有充分证据表明，化疗期间监测抗苗勒管激素可能符合继发性不孕最早诊断指标的标准。
