溶组织内阿米巴的免疫细胞化学检测

Immunocytochemical detection of Entamoeba histolytica.

作者信息

Pérez de Súarez E, Pérez-Schael I, Perozo-Ruggeri G, de Dávila D, Römer H, Tapia F J

机构信息

Escuela de Bionálisis, Facultad de Medicina, Universidad Central de Venezuela, Caracas.

出版信息

Trans R Soc Trop Med Hyg. 1987;81(4):624-6. doi: 10.1016/0035-9203(87)90435-4.

DOI:10.1016/0035-9203(87)90435-4

PMID:2895513

Abstract

Human anti-Entamoeba histolytica immunoglobulin was used to detect Entamoeba histolytica in 74 positive samples from several different sources, using an indirect immunoperoxidase method. In 73 samples, the protozoan was easily identified. Trophozoites and cysts of all cultured Entamoeba strains examined were strongly stained, and as few as 3 trophozoites per microscope slide could be detected. In addition, 51 negative control samples were also tested and non-specific reactions were not observed. These preliminary results show that this method is both sensitive and specific, and can easily detect trophozoites and cysts of different E. histolytica strains.

摘要

采用间接免疫过氧化物酶法，用人抗溶组织内阿米巴免疫球蛋白检测来自几个不同来源的74份阳性样本中的溶组织内阿米巴。在73份样本中，原生动物很容易被识别。所有检测的培养溶组织内阿米巴菌株的滋养体和包囊均被强烈染色，每显微镜载玻片上少至3个滋养体也能被检测到。此外，还检测了51份阴性对照样本，未观察到非特异性反应。这些初步结果表明，该方法既灵敏又特异，能够很容易地检测出不同溶组织内阿米巴菌株的滋养体和包囊。