This IACUC approved study was performed to evaluate the environmental, physiological, and hematological components that contribute to stamina following successive bouts of exercise that included searching (5-min), agility (5-min), and ball retrieve (<10-min). Regularly exercised dogs ( = 12) were evaluated on five separate occasions. The population consisted of eight males and four females ranging in age from 8 to 23 months, which included six Labrador retrievers, three German shepherds, and one each English springer spaniel, German wirehaired pointer, and Dutch shepherd. The exercise period was up to 30 min with 5 min of intermittent rest between the exercise bouts or until a designated trainer determined that the dog appeared fatigued (e.g., curled tongue while panting, seeking shade, or voluntary reluctance to retrieve). At the end of the exercise period, pulse rate (PR), core temperature, blood lactate, and venous blood gas were collected. The median outdoor temperature was 28.9°C (84°F) (IQR; 27.2-30°C/81-86°F) and median humidity was 47% (IQR; 40-57%). Median duration of exercise was 27 min (IQR; 25-29). No dog showed signs of heat stress that required medical intervention. The components used to measure stamina in this study were total activity, post-exercise core body temperature (CBT), and increase in CBT. When controlling for breed, total activity, as measured by omnidirectional accelerometer device, could be predicted from a linear combination of the independent variables: pre-exercise activity ( = 0.008), post-exercise activity ( < 0.001), outdoor temperature ( = 0.005), reduction in base excess in extracellular fluid compartment (BEecf) ( = 0.044), and decrease in TCO ( = 0.005). When controlling for breed and sex, increase in CBT could be predicted from a linear combination of the independent variables: study day ( = 0.005), increase in PR ( < 0.001), increase in lactate ( = 0.001), reduction in BEecf ( = 0.031), increase in glucose ( = 0.044), increase in hematocrit ( = 0.032), and increase in hemoglobin ( = 0.038). This study suggests that the influence of outdoor temperature, pre- and post-exercise activity, and the metabolic parameters are important components of stamina associated with exertion.