Fang Zhuo, Schubert Wolf-Dieter, Gey van Pittius Nicolaas Claudius
DST/NRF Centre of Excellence in Biomedical Tuberculosis Research, US/MRC Centre for Molecular and Cellular Biology, Division of Molecular Biology and Human Genetics, Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, University of Stellenbosch, Francie van Zijl Drive, Tygerberg 7505, South Africa.
Department of Biochemistry, University of Pretoria, cnr Lynnwood Road and Roper Street, Hatfield, Pretoria 0028, South Africa.
Biochem Biophys Rep. 2016 Feb 6;5:448-452. doi: 10.1016/j.bbrep.2016.02.005. eCollection 2016 Mar.
Mycobacteria encode five type VII secretion system (T7SS) or ESX for nutrient acquisition and virulence. Mycosins are membrane-anchored components of ESX with serine protease activity but an unidentified substrate range. Establishing the substrate specificity of individual mycosins will help to elucidate individual ESX functions. Mycosin-1 and -3 orthologues from two environmental mycobacterial species, and , have been heterologously produced, but mycosins from () remain to be studied. Here we describe the successful production of mycosin-3 as a first step in investigating its structure and function.
分枝杆菌编码五种VII型分泌系统（T7SS）或ESX，用于获取营养和致病。霉菌蛋白酶是ESX的膜锚定成分，具有丝氨酸蛋白酶活性，但底物范围不明。确定单个霉菌蛋白酶的底物特异性将有助于阐明单个ESX的功能。来自两种环境分枝杆菌物种的霉菌蛋白酶-1和-3直系同源物已通过异源方式产生，但来自[具体物种]的霉菌蛋白酶仍有待研究。在这里，我们描述了成功生产[具体物种]霉菌蛋白酶-3的过程，这是研究其结构和功能的第一步。
