Finco Alessandra Becker, Machado-de-Ávila Ricardo Andrez, Maciel Rayana, De Moura Juliana, Billiald Philippe, Stinghen Andrea Emilia Marques, Alvarenga Larissa M
Laboratório de Imunoquímica, Departamento de Patologia Básica, Universidade Federal do Paraná, CEP 81531-980 Curitiba, PR, Brazil.
Setor de Ciências da Saúde, Universidade do Extremo Sul Catarinense, CEP88806-000 Criciúma, SC, Brazil.
Biochem Biophys Rep. 2016 Mar 23;6:142-148. doi: 10.1016/j.bbrep.2016.03.011. eCollection 2016 Jul.
Advanced Glycation End Products (AGEs) are toxins that are involved in structural and functional alterations of several organs and tissues, resulting in various pathologies. Several types of AGEs have been described but carboxymethyllysine (CML) is the major antigenic AGE compound. In this study, three different immunogenic carrier proteins (KLH, keyhole limpet hemocyanin; BSA, bovine serum albumin; and HSA, human serum albumin) were modified by glycation. The glycated molecules were used to produce epitope-specific monoclonal antibodies able to recognize the CML domain and to detect uremic toxins in the serum of patients with chronic kidney disease (CKD). A competitive ELISA was standardized in order to quantify CML in the sera of CKD patients. An increase in uremic toxins can compromise the clinical condition of these patients, thus, the detection and quantification of these toxins should contribute to a better management and understanding of this disease.
晚期糖基化终末产物（AGEs）是一类毒素，参与多个器官和组织的结构与功能改变，从而引发各种病理状况。已描述了多种类型的AGEs，但羧甲基赖氨酸（CML）是主要的具有抗原性的AGE化合物。在本研究中，三种不同的免疫原性载体蛋白（钥孔戚血蓝蛋白KLH、牛血清白蛋白BSA和人血清白蛋白HSA）通过糖基化进行了修饰。这些糖基化分子被用于制备能够识别CML结构域并检测慢性肾脏病（CKD）患者血清中尿毒症毒素的表位特异性单克隆抗体。为了定量CKD患者血清中的CML，对竞争性酶联免疫吸附测定（ELISA）进行了标准化。尿毒症毒素的增加会损害这些患者的临床状况，因此，这些毒素的检测和定量应有助于更好地管理和理解这种疾病。
