Wavelength-dependent photocycle activity of xanthorhodopsin in the visible region.

Xanthorhodopsin (xR) is a dual-chromophore proton-pump photosynthetic protein comprising one retinal Schiff base and one light-harvesting antenna salinixanthin (SX). The excitation wavelength-dependent transient population of the intermediate M demonstrates that the excitation of the retinal at 570 nm leads to the highest photocycle activity and the excitations of SX at 460 and 430 nm reduce the activity to 37% relatively, suggesting an energy transfer pathway from the S state of the SX to the S state of the retinal and a quick internal vibrational relaxation in the S state of SX prior to the energy transfer from SX to retinal.