Slouf Václav, Balashov Sergei P, Lanyi Janos K, Pullerits Tõnu, Polívka Tomáš
Institute of Physical Biology, University of South Bohemia, Zámek 136, 373 33 Nové Hrady, Czech Republic.
Chem Phys Lett. 2011 Nov 7;516(1-3):96-101. doi: 10.1016/j.cplett.2011.09.062.
We present a comparative study of xanthorhodopsin, a proton pump with the carotenoid salinixanthin serving as an antenna, and the closely related bacteriorhodopsin. Upon excitation of retinal, xanthorhodopsin exhibits a wavy transient absorption pattern in the region between 470 and 540 nm. We interpret this signal as due to electrochromic effect of the transient electric field of excited retinal on salinixanthin. The spectral shift decreases during the retinal dynamics through the ultrafast part of the photocycle. Differences in dynamics of bacteriorhodopsin and xanthorhodopsin are discussed.
我们展示了对黄视紫红质（一种以类胡萝卜素盐藻黄质作为天线的质子泵）与密切相关的细菌视紫红质的比较研究。在视黄醛被激发后，黄视紫红质在470至540纳米之间的区域呈现出波浪状的瞬态吸收模式。我们将此信号解释为是由于激发态视黄醛的瞬态电场对盐藻黄质的电致变色效应所致。在视黄醛通过光循环的超快部分的动力学过程中，光谱位移减小。讨论了细菌视紫红质和黄视紫红质在动力学方面的差异。
