Anadol Elvan, Yar Saglam Atiye Seda, Gultiken Nilgun, Karakas Kubra, Alcigir Eray, Alkan Hasan, Kanca Halit
1 Gazi University Laboratory, Animal Breeding and Experimental Research Centre, Faculty of Medicine, Gazi University , Besevler, Ankara , Turkey.
2 Department of Medical Biology and Genetics, Faculty of Medicine, Gazi University , Besevler, Ankara , Turkey.
Acta Vet Hung. 2017 Sep;65(3):382-393. doi: 10.1556/004.2017.036.
The aim of the present study was to investigate the relationship between the expression of iNOS, COX-2 and VEGF mRNA levels and malignancy degree in canine malignant mammary tumours. Thirty-five bitches presented with the complaint of mammary masses, aged 6-10 years and representing different breeds, were used. The expressions of iNOS, COX-2 and VEGF mRNA levels were significantly higher in both benign and malignant tumours than in the adjacent nonneoplastic mammary glands (P < 0.05). The iNOS, COX-2 and VEGF mRNA expression levels of grade 2 tumours were higher than those of grade 1 tumours; however, the highest expression levels were detected in grade 3 tumours. Thus, increased iNOS, COX-2 and VEGF gene mRNA levels were found to be related with the histological grade of malignancy in dogs with mammary tumours.
本研究的目的是探讨犬恶性乳腺肿瘤中诱导型一氧化氮合酶（iNOS）、环氧化酶-2（COX-2）和血管内皮生长因子（VEGF）mRNA水平的表达与恶性程度之间的关系。使用了35只出现乳腺肿块主诉的母犬，年龄在6至10岁之间，代表不同品种。iNOS、COX-2和VEGF mRNA水平在良性和恶性肿瘤中的表达均显著高于相邻的非肿瘤性乳腺组织（P < 0.05）。2级肿瘤的iNOS、COX-2和VEGF mRNA表达水平高于1级肿瘤；然而，3级肿瘤中检测到的表达水平最高。因此，发现iNOS、COX-2和VEGF基因mRNA水平的升高与患有乳腺肿瘤的犬的组织学恶性程度相关。
