Zhang Wenlong, Liu Xiaodan, Liu Mengcheng, Ma Bo, Xu Li, Wang Junwei
1 Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University , Harbin, Heilongjiang 150030 , P. R. China.
2 Northeastern Science Inspection Station , China Ministry of Agriculture Key Laboratory of Animal Pathogen Biology , P. R. China.
Acta Vet Hung. 2017 Sep;65(3):327-339. doi: 10.1556/004.2017.032.
Pasteurella multocida, Mannheimia haemolytica and Trueperella pyogenes are three bacterial pathogens closely associated with the bovine respiratory disease complex (BRDC). In the current study, a multiplex PCR for the simultaneous detection of these three bacteria in cultures was established. After serial optimisation, the detection limit of the method for the genomic DNA of the three bacteria was 40 pg/μl. The method could detect the genomic DNA of these three bacteria but not the genomic DNA of seven other bacterial strains. Together with the bacterial enrichment technology, the multiplex PCR could be used for detecting the three bacteria in animal tissues. This method might be valuable for speeding up laboratory diagnosis and directing the treatment of BRDC to these three bacterial pathogens.
多杀性巴氏杆菌、溶血曼氏杆菌和化脓隐秘杆菌是与牛呼吸道疾病综合征（BRDC）密切相关的三种细菌病原体。在本研究中，建立了一种用于同时检测培养物中这三种细菌的多重PCR方法。经过一系列优化后，该方法对这三种细菌基因组DNA的检测限为40 pg/μl。该方法能够检测这三种细菌的基因组DNA，但不能检测其他七种细菌菌株的基因组DNA。结合细菌富集技术，多重PCR可用于检测动物组织中的这三种细菌。该方法对于加快实验室诊断以及指导针对这三种细菌病原体的BRDC治疗可能具有重要价值。
