Yaman Turan, Büyükbayram Hüseyin, Özyıldız Zafer, Terzi Funda, Uyar Ahmet, Keles Ömer Faruk, Özsoy Şule Yurdagül, Yener Zabit
Department of Pathology, Faculty of Veterinary Medicine, Van Yuzuncu Yil University, 65090, Van, Turkey.
Department of Pathology, Faculty of Medicine, Dicle University, 21280, Diyarbakir, Turkey.
J Vet Res. 2018 Dec 31;62(4):439-445. doi: 10.2478/jvetres-2018-0070. eCollection 2018 Dec.
The aim of this study was to determine the predisposing effect of bovine respiratory syncytial virus (BRSV) on spp. infection in naturally-induced pneumonia in cattle by immunohistochemical labelling.
Lungs of cattle slaughtered in the slaughterhouse were examined macroscopically, and 100 pneumonic samples were taken. The samples were fixed in 10% neutral formalin and embedded in paraffin by routine methods. Sections 5 μm in thickness were cut. The streptavidin-peroxidase method (ABC) was used to stain the sections for immuno-histochemical examination.
BRSV antigens were found in the cytoplasm of epithelial cells of bronchi, bronchioles, and alveoles and within inflammatory cell debris and inflammatory exudate in bronchial lumens. spp. antigens were detected in the cytoplasm of the epithelial cells of bronchi and bronchioles, and in cells in the lumens of bronchi and bronchioles. Eleven cases were positive for only one pathogen (six for BRSV and five for spp.), while 35 cases were positive for 2 pathogens: BRSV plus (n = 21) or (n = 14).
The presence of high levels of BRSV in dual infections indicates that BSRV may be the main pneumonia-inducing agent and an important predisposing factor for the formation of spp. infections in cattle naturally afflicted with pneumonia.
本研究的目的是通过免疫组织化学标记来确定牛呼吸道合胞病毒（BRSV）对牛自然诱发肺炎中 spp. 感染的诱发作用。
对在屠宰场宰杀的牛的肺进行宏观检查，并采集100份肺炎样本。样本用10%中性福尔马林固定，按常规方法石蜡包埋。切成5μm厚的切片。采用链霉亲和素-过氧化物酶法（ABC法）对切片进行免疫组织化学染色检查。
在支气管、细支气管和肺泡上皮细胞的细胞质中以及支气管腔内的炎性细胞碎片和炎性渗出物中发现了BRSV抗原。在支气管和细支气管上皮细胞的细胞质以及支气管和细支气管腔内的细胞中检测到了 spp. 抗原。11例仅对一种病原体呈阳性（6例为BRSV阳性，5例为 spp. 阳性），而35例对两种病原体呈阳性：BRSV加 （n = 21）或 （n = 14）。
双重感染中高水平BRSV的存在表明，BRSV可能是主要的致肺炎病原体，也是自然患肺炎牛形成 spp. 感染的重要诱发因素。
