Daniel M D, Letvin N L, Sehgal P K, Schmidt D K, Silva D P, Solomon K R, Hodi F S, Ringler D J, Hunt R D, King N W
New England Regional Primate Research Center, Harvard Medical School, Southborough, MA 01772.
Int J Cancer. 1988 Apr 15;41(4):601-8. doi: 10.1002/ijc.2910410421.
The prevalence of antibodies to 3 retroviruses in the macaque colony of the New England Regional Primate Research Center (NERPRC) was determined using enzyme-linked immunosorbent assay procedures as well as radioimmunoprecipitation-SDS polyacrylamide gel electrophoresis and indirect immunofluorescence tests. Out of 848 macaques, 3 (0.35%) had antibodies to simian immunodeficiency virus (SIV), 27 (3.2%) had antibodies to simian T-lymphotropic virus type I (STLV-1) and approximately 285 (34%) had antibodies to type D retrovirus. Of 3 macaques infected with SIV, 2 were rhesus monkeys (Macaca mulatta) and I was a cynomolgus monkey (Macaca fascicularis). STLV-1 and D retrovirus infection occurred in all 4 macaque species examined. SIV, STLV-1 and D retroviruses were isolated from sero-positive macaques. The low prevalence of SIV infection suggests that SIV is not being readily transmitted among macaques at NERPRC; this contrasts markedly with the high SIV prevalence in some captive mangabey colonies. In contrast to African green monkeys from eastern Africa, 160 Caribbean green monkeys examined showed no sign of SIV infection. These results provide a framework for monitoring spontaneous disease associated with infection by these 3 retroviruses and will help in further definition of STLV-1 and SIV infection of non-human primates as animal models for human disease.
利用酶联免疫吸附测定程序以及放射免疫沉淀-十二烷基硫酸钠聚丙烯酰胺凝胶电泳和间接免疫荧光试验，测定了新英格兰地区灵长类动物研究中心（NERPRC）猕猴群体中3种逆转录病毒抗体的流行情况。在848只猕猴中，3只（0.35%）有抗猴免疫缺陷病毒（SIV）抗体，27只（3.2%）有抗猴I型嗜T淋巴细胞病毒（STLV-1）抗体，约285只（34%）有抗D型逆转录病毒抗体。在感染SIV的3只猕猴中，2只是恒河猴（猕猴属），1只是食蟹猴（食蟹猕猴）。在所检测的所有4种猕猴中均发生了STLV-1和D型逆转录病毒感染。从血清阳性猕猴中分离出了SIV、STLV-1和D型逆转录病毒。SIV感染的低流行率表明SIV在NERPRC的猕猴中不易传播；这与一些圈养白眉猴群体中SIV的高流行率形成了明显对比。与来自东非的非洲绿猴不同，所检测的160只加勒比绿猴没有SIV感染的迹象。这些结果为监测与这3种逆转录病毒感染相关的自发性疾病提供了一个框架，并将有助于进一步明确STLV-1和SIV作为人类疾病动物模型对非人灵长类动物的感染情况。
