铜通过下调Wnt信号通路损害斑马鱼鳔的发育。
Copper impairs zebrafish swimbladder development by down-regulating Wnt signaling.
作者信息
Xu JiangPing, Zhang RuiTao, Zhang Ting, Zhao Guang, Huang Yan, Wang HuanLing, Liu Jing-Xia
机构信息
College of Fisheries, Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, 430070, China.
College of Fisheries, Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, 430070, China; Collaborative Innovation Center for Efficient and Health Production of Fisheries in Hunan Province, Hunan, Changde, 415000, China.
出版信息
Aquat Toxicol. 2017 Nov;192:155-164. doi: 10.1016/j.aquatox.2017.09.018. Epub 2017 Sep 21.
Copper nanoparticles (CuNPs) are used widely in different fields due to their attractive and effective abilities in inhibiting bacteria and fungi, but little information is available about their biological effects and potential molecular mechanisms on fish development. Here, CuNPs and copper (II) ions (Cu) were revealed to inhibit the specification and formation of three layers of zebrafish embryonic posterior swimbladder and impair its inflation in a stage-specific manner. CuNPs and Cu were also revealed to down-regulate Wnt signaling in embryos. Furthermore, Wnt agonist 6-Bromoindirubin-3'-oxime (BIO) was found to neutralize the inhibiting effects of CuNPs or Cu or both on zebrafish swimbladder development. The integrated data here provide the first evidence that both CuNPs and Cu act on the specification and growth of the three layers of swimbladder and inhibit its inflation by down-regulating Wnt signaling in a stage-specific manner during embryogenesis.
铜纳米颗粒（CuNPs）因其在抑制细菌和真菌方面具有吸引力且有效的能力而被广泛应用于不同领域，但关于它们对鱼类发育的生物学效应和潜在分子机制的信息却很少。在此，研究发现CuNPs和铜（II）离子（Cu）会以阶段特异性的方式抑制斑马鱼胚胎后鳔三层结构的特化和形成，并损害其充气过程。研究还发现CuNPs和Cu会下调胚胎中的Wnt信号。此外，还发现Wnt激动剂6-溴靛玉红-3'-肟（BIO）可中和CuNPs或Cu或两者对斑马鱼鳔发育的抑制作用。这里的综合数据首次证明，在胚胎发育过程中，CuNPs和Cu均作用于鳔三层结构的特化和生长，并通过阶段特异性下调Wnt信号来抑制其充气。
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