Graduate School of Pharmaceutical Sciences, Tohoku University, Aramaki, Aoba-ku, Sendai 980-8578, Japan.
Graduate School of Pharmaceutical Sciences, Tohoku University, Aramaki, Aoba-ku, Sendai 980-8578, Japan.
J Colloid Interface Sci. 2018 Jan 15;510:302-307. doi: 10.1016/j.jcis.2017.09.075. Epub 2017 Sep 21.
Multilayer films that decompose in the presence of lactate were prepared by depositing phenylboronic acid-modified poly(allylamine) (PBA-PAH) and poly(vinyl alcohol) (PVA) on a lactate oxidase (LOx) layer. The layers adhered through boronate ester bonds. The resulting LOx(PBA-AH/PVA) film was stable in pH 7.4 solution but decomposed following the addition of lactate. The carbon-boron bonds in PBA residues were cleaved by oxidative reaction with HO produced by the enzymatic reaction of LOx. Approximately 90% of the film decomposed following exposure for 120 and 30min to 0.05 and 20mM lactate at pH 7.4, respectively. The multilayer film therefore decomposed under conditions comparable to the extracellular environment of tumors (20mM lactate at pH 6.5). Our results show that LOx/(PBA-PAH/PVA) multilayer film could be used for cancer drug delivery systems.
通过在层状结构的乳酸氧化酶（LOx）层上沉积经苯硼酸修饰的聚烯丙胺（PBA-PAH）和聚乙烯醇（PVA），制备了可在乳酸存在的条件下分解的多层膜。各层之间通过硼酸酯键连接。在 pH 值为 7.4 的溶液中，所得的 LOx（PBA-PAH/PVA）膜是稳定的，但在加入乳酸后会分解。PBA 残基中的碳-硼键通过 LOx 酶促反应生成的 HO 的氧化反应而被切断。在 pH 值为 7.4 时，分别将薄膜暴露于 0.05 和 20mM 乳酸中 120 和 30min 后，约有 90%的薄膜被分解。因此，该多层膜可在与肿瘤细胞外环境（pH 值 6.5 时 20mM 乳酸）相媲美的条件下分解。我们的研究结果表明，LOx/（PBA-PAH/PVA）多层膜可用于癌症药物传递系统。
