Universidade Federal de Minas Gerais, Instituto de Ciências Biológicas, Departamento de Botânica, Avenida Antônio Carlos, 6627, Pampulha, Caixa Postal 486, 31270-970, Belo Horizonte, Minas Gerais, Brazil; Universidade Federal do Paraná, Setor de Ciências Biológicas, Departamento de Botânica, Avenida Coronel Francisco H. dos Santos, 100, Caixa Postal 19031, Centro Politécnico, 81531-980, Curitiba, Paraná, Brazil.
Fundação Ezequiel Dias, Rua Conde Pereira Carneiro, 80, 30510-010, Belo Horizonte, Minas Gerais, Brazil.
Environ Pollut. 2018 Jan;232:293-299. doi: 10.1016/j.envpol.2017.09.054. Epub 2017 Sep 27.
We investigated the ability of the aquatic fern Azolla to take up ciprofloxacin (Cipro), as well as the effects of that antibiotic on the N-fixing process in plants grown in medium deprived (-N) or provided (+N) with nitrogen (N). Azolla was seen to accumulate Cipro at concentrations greater than 160 μg g dry weight when cultivated in 3.05 mg Cipro l, indicating it as a candidate for Cipro recovery from water. Although Cipro was not seen to interfere with the heterocyst/vegetative cell ratios, the antibiotic promoted changes with carbon and nitrogen metabolism in plants. Decreased photosynthesis and nitrogenase activity, and altered plant's amino acid profile, with decreases in cell N concentrations, were observed. The removal of N from the growth medium accentuated the deleterious effects of Cipro, resulting in lower photosynthesis, N-fixation, and assimilation rates, and increased hydrogen peroxide accumulation. Our results shown that Cipro may constrain the use of Azolla as a biofertilizer species due to its interference with nitrogen fixation processes.
我们研究了水生蕨类植物满江红吸收环丙沙星（Cipro）的能力，以及在缺氮（-N）或提供氮（+N）的培养基中生长的植物中，该抗生素对固氮过程的影响。当在 3.05mg Cipro l 中培养时，满江红积累的 Cipro 浓度大于 160μg g 干重，表明它是从水中回收 Cipro 的候选物。尽管 Cipro 似乎不干扰异形胞/营养细胞的比例，但抗生素促进了植物碳氮代谢的变化。观察到光合作用和固氮酶活性下降，以及细胞氮浓度降低导致植物氨基酸谱改变。从生长培养基中去除氮会加剧 Cipro 的有害影响，导致光合作用、固氮和同化率降低，过氧化氢积累增加。我们的结果表明，由于 Cipro 干扰了固氮过程，它可能会限制满江红作为生物肥料物种的使用。
