The Association of Bovine Osteopontin () Gene with Milk Production Traits in Iranian Holstein Bulls.

BACKGROUND

The Osteopontin () is a highly phosphorylated glycoprotein in numbers of bovine tissues and milk. has been reported to be associated with milk production in cattle.

OBJECTIVE

The genotype and allelic frequencies for and its association with milk production will be evaluated in Iranian Holstein Bulls.

MATERIALS AND METHODS

Bulls DNA (100) was isolated. was used for primer design. Polymerase Chain Reaction was implemented to amplify a 826 bp fragment and the amplicon was digested by . Restricted Maximum likelihood (REML) method based on average information algorithm using ASRMEL programs (version 3.1) was employed to estimate the genetic parameters and variance of components. The association of genotypes with milk production traits were analysed by the least square method as applied in the general linear model (GLM) procedure of SAS. Allele substitution effects were performed by regression analyses.

RESULTS

Allele frequencies of T and C were 0.59±0.03 and 0.41±0.03, respectively. Genotype frequencies of TT, CT and CC were 34.69, 48.62, and 16.69, respectively. The chi-square test showed the deviation from Hardy-Weinberg equilibrium. Estimated heritability for milk yield, fat yield and its percent, protein yield and its percent were 0.28±0.0061, 0.21±0.0064, 0.22±0.0086, 0.32±0.0065 and 0.34±0.0096 respectively. Allelic substitution effects and differences between genotypes were not significant for milk production traits.

CONCLUSIONS

This study suggested that the C allele frequency of was noticeable in Iranian proven bull Holstein population, but was not associated with milk production traits. However, before being practical for the breeding improvement of Iranian Holsteins a larger sample size is required.