Soltani Najafabadi Masood, Abedini Raha, Eskandari Hassan, Mehrabi Rahim
Seed and Plant Improvement Institute (SPII), Karaj, Iran.
Iran J Biotechnol. 2015 Jun;13(2):45-50. doi: 10.15171/ijb.1047.
Downy mildew caused by is a devastating disease in sunflower worldwide. Several dominant resistance genes designated as have been identified and linked molecular markers have been demonstrated. However, no information on theresistance genes is available forIranian lines.
The presence of three map-based molecular markers previously proved to be linked to different resistance genes were evaluated in sunflower inbred lines.
Using PCR-based and CAPS molecular markers, 26 sunflower inbred lines with different responses to race 100 were used to detect the presence of three resistance loci including , and within the lines.
Molecular marker linked to was present in some of the sunflower lines but was not correlated with the phenotypic reaction of the lines to race 100. Despite the use of three markers linked to , PCR failed to amplify any corresponding product. This data may suggest that none of the genotypes possessed locus. -linked cleaved amplified polymorphic sequences (CAPS) were present in several resistance lines and effectively differentiated susceptible and resistant sunflower lines.
Applicability of molecular markers in breeding programs revisited in disease management.
由[病原菌名称未给出]引起的霜霉病是全球向日葵上的一种毁灭性病害。已鉴定出几个被命名为[基因名称未给出]的显性抗性基因，并证明了与之连锁的分子标记。然而，关于伊朗品系的抗性基因尚无信息。
评估先前证明与不同抗性基因连锁的三个基于图谱的分子标记在向日葵自交系中的存在情况。
使用基于PCR的和CAPS分子标记，对26个对霜霉病菌株100有不同反应的向日葵自交系进行检测，以确定这些品系中是否存在包括[基因名称未给出]、[基因名称未给出]和[基因名称未给出]在内的三个抗性位点。
与[基因名称未给出]连锁的分子标记存在于一些向日葵品系中，但与这些品系对菌株100的表型反应无关。尽管使用了与[基因名称未给出]连锁的三个标记，但PCR未能扩增出任何相应产物。该数据可能表明，没有一个基因型拥有[基因名称未给出]位点。与[基因名称未给出]连锁的酶切扩增多态性序列（CAPS）存在于几个抗性品系中，并有效地区分了感病和抗病向日葵品系。
重新审视了分子标记在病害管理育种计划中的适用性。
