头孢噻肟和苄腺嘌呤可促进甜瓜再生。

Cefotaxime and Benzyladenine Improve Melon Regeneration.

作者信息

Naderi Davood, Askari-Khorasgani Omid, Mahmoudi Esmaeil

机构信息

Young Researchers and Elite Club, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran.

Department of Horticulture, Faculty of Agriculture and Natural Resources, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran.

出版信息

Iran J Biotechnol. 2016 Mar;14(1):56-60. doi: 10.15171/ijb.1077.

DOI:10.15171/ijb.1077

PMID:28959319

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5435015/

Abstract

BACKGROUND

Worldwidely cultivated, melon is commercially an important fruit crop, as it is in Iran.

OBJECTIVES

Establishment of an efficient plant regeneration system plays a pivotal role in the plant transformation, hence, the importance of regeneration protocol for Iranian melon (Cucumis melo L. var. Gorgab) has encouraged us to work on melon regeneration.

MATERIAL AND METHODS

The effect of selective media, including various concentrations of the 6-benzyladenine (BA), cefotaxime, as well as indole-3-acetic acid (IAA) on regeneration of the cotyledonary petioles derived from a 6-day-old grown seedlings were assessed.

RESULTS

The highest frequency of regeneration rate was recorded at 1.5 mg.L of the BA plus 250 mg.L cefotaxime in addition to 1 mg.L BA plus 1000 mg.L cefotaxime. The highest percentage of the shoot formation (100%) was recorded at 1 mg.L BA plus 1000 mg.L cefotaxime, while, it was relatively lower (75%) on than the medium containing 1.5 mg.L BA in combination with 250 mg.L cefotaxime. The highest root induction was observed in the medium containing 500 mg.L cefotaxime + 0.1 mg.L IAA. A significant positive influence on roots and leaves formation, as well as their number, in addition to regeneration of shoots was observed as well.

CONCLUSIONS

This is the first work reporting an appropriate regeneration procedure for the melon, an Iranian native crop.

摘要

背景

甜瓜在全球广泛种植，在商业上是一种重要的水果作物，在伊朗也是如此。

目的

建立高效的植物再生系统在植物转化中起着关键作用，因此，伊朗甜瓜（甜瓜变种戈尔加布）再生方案的重要性促使我们开展甜瓜再生研究。

材料与方法

评估了选择性培养基，包括不同浓度的6-苄基腺嘌呤（BA）、头孢噻肟以及吲哚-3-乙酸（IAA）对6日龄幼苗子叶叶柄再生的影响。

结果

在添加1.5mg.L BA加250mg.L头孢噻肟以及1mg.L BA加1000mg.L头孢噻肟的培养基上，再生率最高。在添加1mg.L BA加1000mg.L头孢噻肟的培养基上，芽形成率最高（100%），而在添加1.5mg.L BA与250mg.L头孢噻肟的培养基上相对较低（75%）。在添加500mg.L头孢噻肟+0.1mg.L IAA的培养基上观察到最高的生根率。此外，还观察到对根和叶的形成及其数量以及芽的再生有显著的积极影响。