Matulka R A, Chan T, Green R, Carney J R, Franklin S, Licari P
Burdock Group, 859 Outer Road, Orlando, FL 32814, United States.
Solazyme, Inc., 225 Gateway Blvd., South San Francisco, CA 94080, United States.
Toxicol Rep. 2015 Dec 29;3:123-134. doi: 10.1016/j.toxrep.2015.12.006. eCollection 2016.
Microalgae are increasingly being utilized as food ingredients for a variety of applications, including as sources of protein, egg and dairy substitutes, and cooking oils. The dietary safety of a new structuring fat produced using a heterotrophic fermentation process by a strain of was evaluated in a 13-week dietary toxicity study and compared with kokum fat, a structuring fat of similar composition used in the food industry and derived from seeds. The algal structuring fat was evaluated for its genotoxic potential using both and assays. No treatment-related adverse events occurred in rats consuming algal structuring fat or kokum fat in the 13-week study; no treatment-related effects were reported for body weight, food consumption, urinalysis, hematology, clinical chemistry, gross pathology, organ weights, or histopathology. While statistically significant effects occurred in some parameters, none were dose-related or considered adverse. Overall, the NOAELs for the algal structuring fat and the kokum fat were 100 000 ppm, the highest concentrations tested. The algal structuring fat was not mutagenic in the bacterial reverse mutation assay in the or strains tested and was not clastogenic in the mouse bone marrow chromosome aberration assay.
微藻越来越多地被用作各种应用的食品成分，包括作为蛋白质、鸡蛋和乳制品替代品以及食用油的来源。在一项为期13周的饮食毒性研究中，对一种由 菌株通过异养发酵过程生产的新型结构化脂肪的饮食安全性进行了评估，并与印度紫铆脂进行了比较，印度紫铆脂是食品工业中使用的一种成分相似的结构化脂肪，来源于 种子。使用 和 试验评估了藻类结构化脂肪的遗传毒性潜力。在为期13周的研究中，食用藻类结构化脂肪或印度紫铆脂的大鼠未出现与治疗相关的不良事件；未报告与治疗相关的体重、食物消耗、尿液分析、血液学、临床化学、大体病理学、器官重量或组织病理学影响。虽然某些参数出现了统计学上的显著影响，但均与剂量无关或不被视为不良影响。总体而言，藻类结构化脂肪和印度紫铆脂的无观察到有害作用水平均为100 000 ppm，即测试的最高浓度。在所测试的 或 菌株中，藻类结构化脂肪在细菌回复突变试验中无致突变性，在 小鼠骨髓染色体畸变试验中无染色体断裂作用。
