自发性高血压大鼠和Wistar-Kyoto正常血压大鼠垂体、脑区及外周组织中的阿片肽

Opioid peptides in pituitary gland, brain regions and peripheral tissues of spontaneously hypertensive and Wistar-Kyoto normotensive rats.

作者信息

Bhargava H N, Matwyshyn G A, Hanissian S, Tejwani G A

机构信息

Department of Pharmacodynamics, College of Pharmacy, University of Illinois, Chicago 60612.

出版信息

Brain Res. 1988 Feb 9;440(2):333-40. doi: 10.1016/0006-8993(88)91003-7.

DOI:10.1016/0006-8993(88)91003-7

PMID:2896047

Abstract

The concentrations of beta-endorphin (beta-END), dynorphin (DYN) and methionine-enkephalin (MEK) in pituitary, brain regions, heart, kidney and adrenal of 8 week old male spontaneously hypertensive (SHR) and Wistar-Kyoto (WKY) normotensive rats were determined by radioimmunoassay and compared. The brain regions examined were hypothalamus, striatum, pons + medulla, midbrain and cortex. The concentration of beta-END in pituitary of SHR rats was 49% higher than those of WKY rats. The concentration of beta-END in the striatum of SHR rats was 71% lower as compared to WKY rats. The concentration of beta-END in the heart, adrenals and kidney of SHR rats was significantly lower (92, 48 and 57%, respectively), than those of WKY rat tissues. The concentration of DYN in pituitary, striatum and heart were lower by 38, 55 and 46%, respectively, in SHR compared to WKY rats, but in hypothalamus it was greater (33%) than in WKY rats. The concentration of DYN in other brain areas and in kidney and adrenal did not differ. The tissues of SHR and WKY rats which showed significant difference in the concentration of MEK were pituitary, pons + medulla, cerebral cortex and adrenals. The concentration of MEK was greater in SHR rats with pons + medulla, cortex and adrenals showing 33, 40, 268% higher levels, respectively, over the WKY rat tissues. However, the concentration of MEK in pituitary of SHR rats was 40% lower than that of WKY rats. These studies suggest that the endogenous opioid peptides of both central and peripheral tissues may be important in the regulation of blood pressure in SHR rats.

摘要

采用放射免疫分析法测定并比较了8周龄雄性自发性高血压（SHR）大鼠和Wistar - Kyoto（WKY）正常血压大鼠垂体、脑区、心脏、肾脏和肾上腺中β-内啡肽（β-END）、强啡肽（DYN）和甲硫氨酸脑啡肽（MEK）的浓度。所检测的脑区包括下丘脑、纹状体、脑桥+延髓、中脑和皮层。SHR大鼠垂体中β-END的浓度比WKY大鼠高49%。与WKY大鼠相比，SHR大鼠纹状体中β-END的浓度低71%。SHR大鼠心脏、肾上腺和肾脏中β-END的浓度显著低于WKY大鼠组织（分别低92%、48%和57%）。与WKY大鼠相比，SHR大鼠垂体、纹状体和心脏中DYN的浓度分别降低了38%、55%和46%，但在下丘脑中比WKY大鼠高（33%）。其他脑区以及肾脏和肾上腺中DYN的浓度没有差异。SHR和WKY大鼠组织中MEK浓度存在显著差异的是垂体、脑桥+延髓、大脑皮层和肾上腺。SHR大鼠脑桥+延髓、皮层和肾上腺中MEK的浓度分别比WKY大鼠组织高33%、40%和268%。然而，SHR大鼠垂体中MEK的浓度比WKY大鼠低40%。这些研究表明，中枢和外周组织中的内源性阿片肽可能在SHR大鼠的血压调节中起重要作用。