3B's Research Group - Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4806-909 Taipas, Guimarães, Portugal.
ICVS/3B's - PT Government Associate Laboratory, Braga, Guimarães, Portugal.
J Biomed Mater Res A. 2018 Feb;106(2):479-490. doi: 10.1002/jbm.a.36248. Epub 2017 Oct 24.
Osteoinductive biomaterials represent a promising approach to advance bone grafting. Despite promising, the combination of sustained biodegradability, mechanical strength, and biocompatibility in a unique biomaterial that can also support cell performance and bone formation in vivo is demanding. Herein, we developed gellan gum (GG)-hydroxyapatite (HAp) spongy-like hydrogels to mimic the organic (GG) and inorganic (HAp) phases of the bone. HAp was successfully introduced within the GG polymeric networks, as determined by FTIR and XRD, without compromising the thermostability of the biomaterials, as showed by TGA. The developed biomaterials showed sustained degradation, high swelling, pore sizes between 200 and 300 μm, high porosity (>90%) and interconnectivity (<60%) that was inversely proportional to the total polymeric amount and to CaCl crosslinker. CaCl and HAp reinforced the mechanical properties of the biomaterials from a storage modulus of 40 KPa to 70-80 KPa. This study also showed that HAp and CaCl favored the bioactivity and that cells were able to adhere and spread within the biomaterials up to 21 days of culture. Overall, the possibility to tailor spongy-like hydrogels properties by including calcium as a crosslinker and by varying the amount of HAp will further contribute to understand how these features influence bone cells performance in vitro and bone formation in vivo. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 479-490, 2018.
骨诱导生物材料代表了一种推进骨移植的有前途的方法。尽管很有前途，但在一种独特的生物材料中结合持续的生物降解性、机械强度和生物相容性，同时还能支持细胞性能和体内骨形成，这是具有挑战性的。在此，我们开发了结冷胶（GG）-羟基磷灰石（HAp）海绵状水凝胶，以模拟骨的有机（GG）和无机（HAp）相。通过 FTIR 和 XRD 确定 HAp 成功地引入到 GG 聚合物网络中，而生物材料的热稳定性没有受到影响，如 TGA 所示。所开发的生物材料表现出持续的降解、高溶胀、200-300μm 之间的孔径、高孔隙率（>90%）和连通性（<60%），这与总聚合物量和 CaCl 交联剂成反比。CaCl 和 HAp 增强了生物材料的机械性能，从储存模量 40kPa 增加到 70-80kPa。这项研究还表明，HAp 和 CaCl 有利于生物活性，并且细胞能够在生物材料中粘附和扩展，培养时间长达 21 天。总之，通过将钙作为交联剂并通过改变 HAp 的量来定制海绵状水凝胶的特性，将有助于进一步了解这些特性如何影响体外骨细胞的性能和体内骨的形成。©2017Wiley 期刊，Inc. J 生物医学材料研究部分 A：106A：479-490，2018。
