3B Research Group-Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Avepark-Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Barco GMR, Portugal. ICVS/3B-PT Government Associated Laboratory, Braga, Portugal.
Biomed Mater. 2018 Mar 16;13(3):035012. doi: 10.1088/1748-605X/aaaf29.
Bone tissue engineering with cell-scaffold constructs has been attracting a lot of attention, in particular as a tool for the efficient guiding of new tissue formation. However, the majority of the current strategies used to evaluate novel biomaterials focus on osteoblasts and bone formation, while osteoclasts are often overlooked. Consequently, there is limited knowledge on the interaction between osteoclasts and biomaterials. In this study, the ability of spongy-like gellan gum and hydroxyapatite-reinforced gellan gum hydrogels to support osteoclastogenesis was investigated in vitro. First, the spongy-like gellan gum and hydroxyapatite-reinforced gellan gum hydrogels were characterized in terms of microstructure, water uptake and mechanical properties. Then, bone marrow cells isolated from the long bones of mice and cultured in spongy-like hydrogels were treated with 1,25-dihydroxyvitamin D3 to promote osteoclastogenesis. It was shown that the addition of HAp to spongy-like gellan gum hydrogels enables the formation of larger pores and thicker walls, promoting an increase in stiffness. Hydroxyapatite-reinforced spongy-like gellan gum hydrogels support the formation of the aggregates of tartrate-resistant acid phosphatase-stained cells and the expression of genes encoding DC-STAMP and Cathepsin K, suggesting the differentiation of bone marrow cells into pre-osteoclasts. The hydroxyapatite-reinforced spongy-like gellan gum hydrogels developed in this work show promise for future use in bone tissue scaffolding applications.
细胞-支架构建物的骨组织工程一直受到广泛关注，特别是作为有效引导新组织形成的工具。然而，目前用于评估新型生物材料的大多数策略都集中在成骨细胞和骨形成上，而破骨细胞往往被忽视。因此，人们对破骨细胞与生物材料之间的相互作用知之甚少。在这项研究中，研究了海绵状凝胶多糖和羟基磷灰石增强型凝胶多糖水凝胶在体外支持破骨细胞生成的能力。首先，从小鼠长骨中分离出骨髓细胞，并在海绵状水凝胶中培养，然后用 1,25-二羟基维生素 D3 处理以促进破骨细胞生成。结果表明，将 HAp 添加到海绵状凝胶多糖水凝胶中可以形成更大的孔和更厚的壁，从而增加刚度。羟基磷灰石增强型海绵状凝胶多糖水凝胶支持抗酒石酸酸性磷酸酶染色细胞聚集的形成和编码 DC-STAMP 和组织蛋白酶 K 的基因的表达，表明骨髓细胞向破骨前体细胞分化。本研究中开发的羟基磷灰石增强型海绵状凝胶多糖水凝胶有望在未来的骨组织支架应用中得到应用。
