一种在胚胎发生过程中表达的斑马鱼engrailed样同源框序列。

A zebrafish engrailed-like homeobox sequence expressed during embryogenesis.

作者信息

Fjose A, Eiken H G, Njølstad P R, Molven A, Hordvik I

机构信息

Laboratory for Biotechnology, University of Bergen, Norway.

出版信息

FEBS Lett. 1988 Apr 25;231(2):355-60. doi: 10.1016/0014-5793(88)80849-4.

DOI:10.1016/0014-5793(88)80849-4

PMID:2896133

Abstract

The zebrafish genome was found to contain two sequences which cross-hybridize strongly with the engrailed gene of Drosophila. Several independent clones containing one of these cross-hybridizing sequences were isolated from a zebrafish genomic library. Characterization of this region (ZF-EN) by DNA sequencing showed that it shares about 70% sequence identity with the engrailed homeobox. More extensive homeobox homology (greater than 90%) was found relative to the murine En genes. The closest relationship exists between ZF-EN and En-2 where the C-terminal domains (104 amino acids) encoded by these genes are almost identical. We also observed that ZF-EN and En-2 are very similar with respect to their transcript sizes and temporal expression patterns.

摘要

人们发现斑马鱼基因组包含两个与果蝇的engrailed基因强烈交叉杂交的序列。从斑马鱼基因组文库中分离出了几个包含其中一个交叉杂交序列的独立克隆。通过DNA测序对该区域（ZF-EN）进行表征表明，它与engrailed同源异型框的序列同一性约为70%。相对于小鼠的En基因，发现了更广泛的同源异型框同源性（大于90%）。ZF-EN与En-2之间存在最密切的关系，这些基因编码的C末端结构域（104个氨基酸）几乎相同。我们还观察到，ZF-EN和En-2在转录本大小和时间表达模式方面非常相似。