Poole S J, Law M L, Kao F T, Lau Y F
Departments of Biochemistry, Howard Hughes Medical Institute, University of California, San Francisco 94143.
Genomics. 1989 Apr;4(3):225-31. doi: 10.1016/0888-7543(89)90324-8.
By low stringency hybridization we have isolated from a human cosmid genomic library sequences homologous with a probe from the Drosophila engrailed gene. Partial nucleotide sequence analysis shows a consensus splice acceptor site followed by an open reading frame (ORF) that can encode 104 amino acids; the first 94 amino acids have 71% identity with the Drosophila engrailed protein. The shared region contains a homeo domain and is within the region of engrailed shared with the Drosophila invected gene and the mouse En-1 and En-2 genes. At the amino acid level, the human sequence is 85% identical with the mouse En-1 gene and 100% identical with the mouse En-2 gene. Hybridization against a panel of human-hamster somatic cell hybrids maps this human En-2 gene to chromosome 7, and regional mapping by in situ hybridization to human chromosomes localizes it to region 7q36 at the end of the long arm.
通过低严格度杂交，我们从一个人类黏粒基因组文库中分离出了与果蝇engrailed基因探针同源的序列。部分核苷酸序列分析显示，有一个共有剪接受体位点，其后是一个可编码104个氨基酸的开放阅读框（ORF）；前94个氨基酸与果蝇engrailed蛋白有71%的同一性。共享区域包含一个同源异型结构域，并且位于与果蝇invected基因以及小鼠En-1和En-2基因共享的engrailed区域内。在氨基酸水平上，人类序列与小鼠En-1基因有85%的同一性，与小鼠En-2基因有100%的同一性。与一组人类-仓鼠体细胞杂种进行杂交，将这个人En-2基因定位到7号染色体上，通过原位杂交对人类染色体进行区域定位，将其定位到长臂末端的7q36区域。
