Levinsky H, Allalouf D, Sagiv M, Barnet M, Singer R
Male Fertility Laboratory, Beilinson Medical Center, Petah Tikva, Israel.
Int J Fertil. 1988 Jan-Feb;33(1):68-71.
Sialic acid (SA) has been estimated in human spermatozoa separated according to age by the use of a discontinuous gradient of human serum albumin (HSA), and according to quality (viability and motility) by the use of a discontinuous gradient of bovine serum albumin (BSA). The amount of SA in the "mature" sperm was 3.5 +/- 0.7 (SD) micrograms/mg protein, whereas in the immature sperm subpopulation, enriched with morphologically abnormal sperm, it was 6.9 +/- 3.4 micrograms/mg protein (P = .05). SA estimated in spermatozoa separated according to quality had values of 3.6 +/- 1.2 micrograms/mg protein in sperm of higher quality and 4.1 +/- 1.3 micrograms/mg protein in that of lower quality; this difference was not of statistical significance. It is suggested that changes in SA levels might reflect differences in the degree of sialylation of protein in the subpopulation of sperm, and thus be related to various stages in the fertilization process.
已通过使用人血清白蛋白（HSA）不连续梯度，根据年龄对人精子中的唾液酸（SA）进行了估算，并通过使用牛血清白蛋白（BSA）不连续梯度，根据质量（活力和运动性）对其进行了估算。“成熟”精子中的SA含量为3.5±0.7（标准差）微克/毫克蛋白质，而在富含形态异常精子的未成熟精子亚群中，其含量为6.9±3.4微克/毫克蛋白质（P = 0.05）。根据质量分离的精子中估算的SA，高质量精子中的值为3.6±1.2微克/毫克蛋白质，低质量精子中的值为4.1±1.3微克/毫克蛋白质；这种差异无统计学意义。有人提出，SA水平的变化可能反映了精子亚群中蛋白质唾液酸化程度的差异，因此与受精过程的各个阶段有关。
