Integrative Parasitology, Center for Infectious Diseases, Heidelberg University Medical School, Im Neuenheimer Feld 324, 69120 Heidelberg, Germany.
Max Planck Institute of Biophysics, Max-von-Laue Strasse 3, 60438 Frankfurt, Germany.
FEMS Microbiol Rev. 2017 Nov 1;41(6):828-853. doi: 10.1093/femsre/fux041.
In recent years, novel imaging approaches revolutionised our understanding of the cellular and molecular biology of microorganisms. These include advances in fluorescent probes, dynamic live cell imaging, superresolution light and electron microscopy. Currently, a major transition in the experimental approach shifts electron microscopy studies from a complementary technique to a method of choice for structural and functional analysis. Here we review functional insights into the molecular architecture of viruses, bacteria and parasites as well as interactions with their respective host cells gained from studies using cryogenic electron tomography and related methodologies.
近年来，新型成像方法彻底改变了我们对微生物的细胞和分子生物学的理解。这些方法包括荧光探针的进步、动态活细胞成像、超高分辨率光和电子显微镜。目前，实验方法的重大转变将电子显微镜研究从一种补充技术转变为结构和功能分析的首选方法。在这里，我们回顾了使用低温电子断层扫描和相关方法获得的有关病毒、细菌和寄生虫的分子结构以及它们与各自宿主细胞相互作用的功能见解。
