Li Qian, Peng Jie, Liu Ting, Zhang Guiying
Department of Gastroenterology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
Exp Ther Med. 2017 Sep;14(3):1935-1940. doi: 10.3892/etm.2017.4769. Epub 2017 Jul 11.
Fas, which is an apoptotic-related protein, has an important role in cell apoptosis. Fas ligand (FasL) binds to Fas and activates apoptosis signal transduction. We previously demonstrated that the efficiency of celecoxib inhibited the proliferation and apoptosis of HT-29 colon cancer cell line. The BGC823 cell line was used as an experimental model to evaluate the potential role of celecoxib on gastric cancer cell apoptosis. Inhibitory effects of celecoxib on cell viability were determined by MTT assay. Cell apoptosis was evaluated by flow cytometric analysis and laser confocal microscopy. The results of the present study demonstrated that celecoxib inhibited the viability of BGC823 cells in a concentration- and time-dependent manner. Furthermore, the effect of BGC823 cells apoptosis was increased in a concentration-dependent manner. Western blotting was used to determine the protein expression levels of Fas, FasL, and B-cell lymphoma-2 (Bcl-2). During the celecoxib-induced apoptosis of BGC823 cells, celecoxib upregulated Fas expression and downregulated FasL and Bcl-2 expression in a concentration-dependent manner. These results suggest that celecoxib inhibited the growth and induced apoptosis of BGC823 gastric cancer cells by regulating the protein expression of Fas, FasL and Bcl-2.
Fas是一种与细胞凋亡相关的蛋白质，在细胞凋亡中起重要作用。Fas配体（FasL）与Fas结合并激活凋亡信号转导。我们之前证明塞来昔布的作用效率可抑制HT - 29结肠癌细胞系的增殖和凋亡。本研究以BGC823细胞系作为实验模型，评估塞来昔布对胃癌细胞凋亡的潜在作用。通过MTT法测定塞来昔布对细胞活力的抑制作用。采用流式细胞术分析和激光共聚焦显微镜评估细胞凋亡情况。本研究结果表明，塞来昔布以浓度和时间依赖性方式抑制BGC823细胞的活力。此外，BGC823细胞凋亡效应呈浓度依赖性增加。采用蛋白质免疫印迹法检测Fas、FasL和B细胞淋巴瘤 - 2（Bcl - 2）的蛋白表达水平。在塞来昔布诱导BGC823细胞凋亡过程中，塞来昔布以浓度依赖性方式上调Fas表达，下调FasL和Bcl - 2表达。这些结果表明，塞来昔布通过调节Fas、FasL和Bcl - 2的蛋白表达来抑制BGC823胃癌细胞的生长并诱导其凋亡。
