Liu Guodong, Liu Zhenfang, Yan Yuexiang, Wang Haiyan
Department of General Surgery, Yidu Central Hospital of Weifang, Weifang, Shandong 262500, P.R. China.
Department of General Surgery, Weifang People's Hospital, Weifang, Shandong 261041, P.R. China.
Oncol Lett. 2017 Dec;14(6):7374-7378. doi: 10.3892/ol.2017.7143. Epub 2017 Oct 5.
The aim of the present study was to examine the effect of fraxetin on proliferation and apoptosis in the MCF-7 breast cancer cell line. Cell proliferation was measused using an MTT assay and 4',6-diamidino-2-phenylindole (DAPI) staining was used to determine shrinkage and condensation. RT-PCR was used to examine the expression of factor-associated suicide () and Fas ligand () mRNA, and western blot analysis was used to examine Bax and Bcl-2 protein. MTT showed that the proliferation of MCF-7 cells was significantly inhibited by fraxetin in a dose-dependent manner. Fraxetin also induced significant morphological changes of MCF-7 cells, suggestive of apoptosis, whereas DAPI staining showed that fraxetin caused cell shrinkage and chromatin condensation. RT-PCR showed that the expression of and mRNA was upregulated by fraxetin and the western blot analysis revealed that Bax was upregulated and Bcl-2 was downregulated. In conclusion, fraxetin can inhibit the proliferation of MCF-7 cells, induce apoptosis, upregulate and Bax, and downregulate Bcl-2 to induce apoptosis. These results support the potential therapeutic role for fraxetin in breast cancer.
本研究的目的是检测紫铆亭对MCF-7乳腺癌细胞系增殖和凋亡的影响。采用MTT法检测细胞增殖，并用4',6-二脒基-2-苯基吲哚（DAPI）染色来确定细胞皱缩和凝聚情况。采用逆转录聚合酶链反应（RT-PCR）检测因子相关自杀基因（Fas）和Fas配体（FasL）mRNA的表达，并用蛋白质免疫印迹分析检测Bax和Bcl-2蛋白。MTT结果显示，紫铆亭能以剂量依赖的方式显著抑制MCF-7细胞的增殖。紫铆亭还可诱导MCF-7细胞发生明显的形态学变化，提示细胞凋亡，而DAPI染色显示紫铆亭可导致细胞皱缩和染色质凝聚。RT-PCR结果显示，紫铆亭可上调Fas和FasL mRNA的表达，蛋白质免疫印迹分析显示Bax表达上调，Bcl-2表达下调。总之，紫铆亭可抑制MCF-7细胞增殖，诱导细胞凋亡，上调Fas和Bax表达，下调Bcl-2表达以诱导细胞凋亡。这些结果支持紫铆亭在乳腺癌治疗中的潜在作用。
