Centre d'Immunologie de Marseille-Luminy (CIML), Aix-Marseille University, UM2, Institut National de la Santé et de la Recherche Médicale (INSERM), U1104, Centre National de la Recherche Scientifique (CNRS), UMR7280, Parc Scientifique et Technologique de Luminy, Case 906, 13288 Marseille, France.
Laboratoire Adhesion & Inflammation, UMR INSERM 1067, UMR CNRS 7333, Aix-Marseille Université Case 937, CEDEX 09, 13288 Marseille, France.
Toxins (Basel). 2021 Sep 8;13(9):632. doi: 10.3390/toxins13090632.
Whooping cough is a severe, highly contagious disease of the human respiratory tract, caused by . The pathogenicity requires several virulence factors, including toxin (PTX), a key component of current available vaccines. Current vaccines do not induce mucosal immunity. Tissue-resident memory T cells (Trm) are among the first lines of defense against invading pathogens and are involved in long-term protection. However, the factors involved in Trm establishment remain unknown. Comparing two strains expressing PTX (WT) or not (ΔPTX), we show that the toxin is required to generate both lung CD4 and CD8 Trm. Co-administering purified PTX with ΔPTX is sufficient to generate these Trm subsets. Importantly, adoptive transfer of lung CD4 or CD8 Trm conferred protection against in naïve mice. Taken together, our data demonstrate for the first time a critical role for PTX in the induction of mucosal long-term protection against .
百日咳是一种严重的、高度传染性的人类呼吸道疾病，由 引起。致病性需要几种毒力因子，包括 毒素 (PTX)，这是当前可用疫苗的关键组成部分。目前的疫苗不能诱导黏膜免疫。组织驻留记忆 T 细胞 (Trm) 是抵御入侵病原体的第一道防线之一，参与长期保护。然而，参与 Trm 建立的因素尚不清楚。比较表达 PTX（WT）或不表达（ΔPTX）的两种 株，我们表明毒素是产生肺部 CD4 和 CD8 Trm 所必需的。同时给予纯化的 PTX 和 ΔPTX 足以产生这些 Trm 亚群。重要的是，肺 CD4 或 CD8 Trm 的过继转移赋予了对 感染的保护作用。总之，我们的数据首次证明了 PTX 在诱导针对 的黏膜长期保护中的关键作用。
