Cowburn R, Hardy J, Roberts P, Briggs R
Department of Biochemistry and Molecular Biology, St. Mary's Hospital Medical School, London, U.K.
Neurosci Lett. 1988 Mar 21;86(1):109-13. doi: 10.1016/0304-3940(88)90192-9.
Sodium dependent D-aspartate and Ca2+/Cl-independent L-glutamate binding assays were used to assess the integrity of glutamate uptake sites and postsynaptic NMDA receptor levels in control and Alzheimer's disease temporal cortex. The number of glutamate uptake sites was significantly and substantially reduced in accordance with previous findings. However, NMDA receptor levels were unchanged. This finding suggests that glutamatergic presynaptic elements are severely reduced in Alzheimer's disease, whilst postsynaptic elements remain intact.
采用钠依赖性D-天冬氨酸和钙/氯非依赖性L-谷氨酸结合试验，评估对照和阿尔茨海默病颞叶皮质中谷氨酸摄取位点的完整性和突触后N-甲基-D-天冬氨酸（NMDA）受体水平。与之前的研究结果一致，谷氨酸摄取位点的数量显著且大幅减少。然而，NMDA受体水平未发生变化。这一发现表明，在阿尔茨海默病中，谷氨酸能突触前元件严重减少，而突触后元件保持完整。
